Chemopreventive activity of polyphenolics from black Jamapa bean (Phaseolus vulgaris L.) on HeLa and HaCaT cells

Journal of Agricultural and Food Chemistry
Xochitl Aparicio-FernándezGuadalupe Loarca-Pina

Abstract

The antiproliferative effects of 100% methanol crude extract and of Toyopearl and silica gel fractions from the seed coats of black Jamapa beans (Phaseolus vulgaris L.) were evaluated using HeLa, human adenocarcinoma cells, and HaCaT, human premalignant keratinocytes. The 100% methanol crude extract [172.2 microM equiv of (+)-catechin] increased adhesion of HeLa cells; however, 3- and 5-fold higher concentrations decreased the number of cells attached as a function of the treatment time. The highest concentration tested diminished the cell adhesion until 40% (after 24 h) to almost 80% (after 72 h). The IC50 values showed that the 100% methanol crude extract was the most effective inhibitor of HeLa cell proliferation, even when it was dissolved in dimethylsulfoxide (DMSO) [34.5 microM equiv of (+)-catechin] or in medium [97.7 microM equiv of (+)-catechin]. The Toyopearl 5 (TP5) fraction and silica gel 2 (SG2) fraction inhibited 60% of the HeLa cell proliferation. The IC50 was 154 microM equiv of (+)-catechin of the 100% methanol crude extract on HaCaT cells. Toyopearl fractions TP4 and TP6 significantly inhibited HaCaT cell proliferation, but the silica gel fractions did not have a significant effect. The 100% methanol crude ext...Continue Reading

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Citations

Dec 21, 2007·Plant Foods for Human Nutrition·Xochitl Aparicio-FernándezGuadalupe Loarca-Pina
Aug 19, 2009·Plant Foods for Human Nutrition·R Mora-EscobedoRafael Reza-Alemán
Nov 30, 2011·Immunopharmacology and Immunotoxicology·Hyung-In MoonJai-Heon Lee
Jun 12, 2013·Bioscience, Biotechnology, and Biochemistry·Satoko ShinjoMasaya Imoto
Mar 1, 2008·Food Chemistry·Long-Ze LinDevanand L Luthria
Dec 12, 2007·Journal of Applied Toxicology : JAT·Christopher D LindsayJacques D Mathieu
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Nov 6, 2013·Critical Reviews in Food Science and Nutrition·Francesc PuiggròsLluís Arola
Nov 23, 2013·Critical Reviews in Food Science and Nutrition·Imran HayatShaukat Bashir
Feb 23, 2011·Food Science and Technology International = Ciencia Y Tecnología De Los Alimentos Internacional·M Reyes-BastidasC Reyes-Moreno
Nov 9, 2017·International Journal of Molecular Sciences·Kumar Ganesan, Baojun Xu
Aug 8, 2018·Diseases·Shiwangni RaoChristopher L Blanchard
Aug 18, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Celia Chávez-Mendoza, Esteban Sánchez
Mar 15, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Tiziana EspositoFrancesca Sansone
Nov 8, 2007·Annals of the New York Academy of Sciences·Mary Ann Lila
Nov 1, 2018·Comprehensive Reviews in Food Science and Food Safety·Qiong-Qiong YangHarold Corke
Nov 6, 2008·Journal of Agricultural and Food Chemistry·José Moisés LaparraDennis D Miller
Jan 4, 2007·Journal of Agricultural and Food Chemistry·Lena Galvez RanillaFranco Maria Lajolo

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