Apr 24, 2018

Chemoselective labeling and site-specific mapping of 5-formylcytosine as a cellular nucleic acid modification

FEBS Letters
Julia DietzschClaudia Höbartner

Abstract

DNA methylation has a profound impact on the regulation of gene expression in normal cell development, and aberrant methylation has been recognized as a key factor in the pathogenesis of human diseases such as cancer. The discovery of modified nucleobases arising from 5-methylcytosine (5mC) through consecutive oxidation to give 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC), and 5-carboxylcytosine (5caC) has stimulated intense research efforts regarding the biological functions of these epigenetic marks. This Review focuses on the sensitive detection and quantitation of 5fC in DNA and RNA by chemoselective labeling, which aims at discriminating between 5fC and its thymine counterpart 5-formyluracil (5fU), and summarizes single-base resolution sequencing methods for locus-specific mapping of 5mC and its oxidized derivatives.

  • References103
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Citations

Mentioned in this Paper

Pathogenic Aspects
DNA Methylation [PE]
Research
Laboratory Procedures
Nucleic Acid Sequence Based Analysis
Pathogenesis
Derivatives
Protein Methylation
Regulation of Biological Process
Cytosine

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