PMID: 3767349Jan 1, 1986Paper

Chemostat enrichment and isolation of Hyphomicrobium EG. A dimethyl-sulphide oxidizing methylotroph and reevaluation of Thiobacillus MS1

Antonie van Leeuwenhoek
G M Suylen, J G Kuenen

Abstract

A stable mixed bacterial culture was obtained by chemostat enrichment using dimethyl-sulphoxide as a carbon and energy source. This culture could not only rapidly oxidize dimethyl-sulphoxide but also dimethyl-sulphide. Enzyme determinations indicated that an important part of it consisted of methylotrophs, which assimilated carbon via the serine pathway. Indeed plate counts revealed the majority of the community to be a Hyphomicrobium species. This organism, designated Hyphomicrobium EG, is an obligate methylotroph which can only grow aerobically on several different C1-compounds. Its performance on dimethyl-sulphoxide was compared with that of the community and of another recently isolated strain, Hyphomicrobium S. The mixed culture, Hyphomicrobium EG and Hyphomicrobium S had a mu max of 0.08, 0.08 and 0.014 h-1 respectively. The KS for dimethyl-sulphoxide was the same for all three cultures (3-6 microM), whereas that for dimethyl-sulphide of Hyphomicrobium EG after growth on dimethyl-sulphoxide was 3-fold higher than that of the other two cultures (48 and 16 microM respectively). After growth on dimethyl-sulphide it improved to 3 microM. Dimethyl-sulphide respiration was maximal at a concentration of 100 microM; higher concen...Continue Reading

References

Dec 1, 1976·Biotechnology and Bioengineering·I GoldbergR I Mateles
Sep 1, 1975·The Biochemical Journal·R B Cox, J R Quayle
Jan 14, 1983·Biochemical and Biophysical Research Communications·S S MhatreD S Pradhan
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Feb 1, 1957·Biochimica Et Biophysica Acta·B SORBO
Sep 1, 1957·Bacteriological Reviews·W VISHNIAC, M SANTER
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Citations

Jan 1, 1992·Applied Biochemistry and Biotechnology·R S TiwareeM Shoda
Nov 8, 2006·Applied Microbiology and Biotechnology·Arjan PolHuub J M Op den Camp
Oct 20, 2005·Journal of Bioscience and Bioengineering·Kuniki KinoKohtaro Kirimura
Aug 30, 2008·The ISME Journal·Suzanne C M HaaijerHuub J M Op den Camp
Jun 27, 2001·Applied and Environmental Microbiology·C G FriedrichJ Fischer
Oct 20, 2005·Journal of Bioscience and Bioengineering·Takako Murakami-NittaShoji Usami
Oct 20, 2005·Journal of Bioscience and Bioengineering·Takako Murakami-NittaKuniki Kino
Jun 1, 1991·Applied and Environmental Microbiology·P T VisscherH van Gemerden

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