Chemotactic activity of tears and bacteria isolated during adverse responses

Experimental Eye Research
A Thakur, M D Willcox

Abstract

Inflammatory processes are characterized by the dynamic influx of leukocytes. This leukocyte recruitment and activation is thought to be initiated by chemical signals including chemotactic factors. This study was designed to investigate the chemotactic activity in different tear types and bacteria isolated during adverse responses to contact lens wear. Chemotactic activity was determined by quantitating in vitro neutrophil migration using a microchemotaxis chamber. Results demonstrated that tears collected immediately after 8 hours sleep (P<0.001) and tears collected during adverse responses (P<0.001) showed significantly higher chemotactic activity compared to reflex tears. Specific neutralizing antibodies to IL-8, LTB4 and C5a were added to closed eye and adverse response tears. Pre-incubation of closed eye tears with antibodies to IL-8 showed a significant reduction in chemotactic activity (P<0.0001), whereas a significant reduction of PMN migration in adverse response tears was observed after pre-treatment with antibodies to LTB4 (P<0.0001). However no difference in chemotactic activity was observed after incubation with antibody to C5a or irrelevant antibody. Dot blots demonstrated that closed eye tears contained approxima...Continue Reading

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Citations

Feb 15, 2012·The Ocular Surface·Andre GrixtiAmit Vikram Datta
Jun 27, 2006·Progress in Histochemistry and Cytochemistry·Friedrich P Paulsen, Monica S Berry
Mar 14, 2000·Experimental Eye Research·A Thakur, M D Willcox
Apr 1, 2012·Saudi Journal of Ophthalmology : Official Journal of the Saudi Ophthalmological Society·Abdullah G Al-Otaibi
Dec 17, 2002·Clinical & Experimental Optometry : Journal of the Australian Optometrical Association·Mark DP Willcox, Jenny Lan
Nov 30, 2004·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·F LallemandR Gurny
Aug 18, 2017·Optometry and Vision Science : Official Publication of the American Academy of Optometry·Simin MasoudiMark D P Willcox
Jul 16, 2003·Eye & Contact Lens·Marc W RobboyCarolyn M Kalsow
Oct 19, 2004·Cell and Tissue Research·Marie-Laure Rachel AkninDoreen Khan-Lim
May 11, 2006·American Journal of Physiology. Lung Cellular and Molecular Physiology·Sheridan HennessAlaina J Ammit
May 24, 2019·Clinical & Experimental Optometry : Journal of the Australian Optometrical Association·Mark Dp Willcox
Apr 10, 2019·Current Medicinal Chemistry·Alberto López-de la RosaAmalia Enríquez-de-Salamanca
Dec 22, 2016·Eye & Contact Lens·Mark D P Willcox
Mar 13, 2002·Paediatric Drugs·Serina StrettonMark D P Willcox
Jan 17, 2012·American Journal of Physiology. Lung Cellular and Molecular Physiology·Melanie ManetschAlaina J Ammit
Jan 21, 2021·Contact Lens & Anterior Eye : the Journal of the British Contact Lens Association·Maria K WalkerStephen J Vincent

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