Chemotactic and osmotic signals share a cGMP transduction pathway in Dictyostelium discoideum

FEBS Letters
H Kuwayama, Peter J M Van Haastert

Abstract

In the ameboid eukaryote Dictyostelium discoideum, chemotactic stimulation by cAMP induces an increase of intracellular cGMP and subsequently the phosphorylation of myosin heavy chain II. Resistance to high osmotic stress also requires transient increases of intracellular cGMP and phosphorylation of myosin heavy chain II, although the kinetics is much slower than for chemotaxis. To examine if chemotaxis and osmotic stress share common signaling components we systematically analyzed the osmotic cGMP response and survival in chemotactic mutants with altered cGMP signaling. Null mutants with deletions of cell surface cAMP receptors or the associated GTP-binding proteins Galpha2 and Gbeta show no cAMP-induced cGMP response and chemotaxis; in contrast, osmotic stress induces the normal cGMP accumulation and survival. The same result was obtained with the non-chemotactic mutant KI-10, which lacks the activation of guanylyl cyclase by cAMP. This indicates that these components are required for chemotaxis but not osmotic cGMP signaling and survival. The potential guanylyl cyclase null mutant KI-8 shows no chemotaxis, no osmotic cGMP increase and reduced survival in high osmolarity. Two types of cGMP-binding protein mutants, KI-4 and KI...Continue Reading

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Citations

Oct 24, 2003·Biochimica Et Biophysica Acta·Douwe M VeltmanPeter J M Van Haastert
Mar 10, 2001·The Biochemical Journal·J RoelofsP J Van Haastert
Jul 23, 2002·Molecular Biology of the Cell·Alexandre TaminatoGlaucia Mendes Souza
Dec 17, 2004·Molecular Biology of the Cell·Douwe M VeltmanPeter J M Van Haastert
Apr 28, 2011·Development, Growth & Differentiation·Richard H GomerDerrick Brazill
Jul 22, 2011·Journal of Signal Transduction·Tong Sun, Leung Kim
Oct 8, 2009·Journal of Cell Science·Anna BagordaCarole A Parent
Oct 19, 2013·Integrative Biology : Quantitative Biosciences From Nano to Macro·Eva K B PfannesCarsten Beta
Jun 5, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Ignacio MarínPeter J M van Haastert
Jun 19, 2003·Protist·Douwe Veltman, Peter J M Van Haastert

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