PMID: 8955292Dec 1, 1996Paper

Chemotactic signaling by the P1 phosphorylation domain liberated from the CheA histidine kinase of Escherichia coli

Journal of Bacteriology
A Garzón, J S Parkinson

Abstract

CheA is a histidine kinase central to the signal transduction pathway for chemotaxis in Escherichia coli. CheA autophosphorylates at His-48, with ATP as the phosphodonor, and then donates its phosphoryl groups to two aspartate autokinases, CheY and CheB. Phospho-CheY controls the flagellar motors, whereas phospho-CheB participates in sensory adaptation. Polypeptides encompassing the N-terminal P1 domain of CheA can be transphosphorylated in vitro by the CheA catalytic domain and yet have no deleterious effect on chemotactic ability when expressed at high levels in wild-type cells. To find out why, we examined the effects of a purified P1 fragment, CheA[1-149], on CheA-related signaling activities in vitro and devised in vivo assays for those same activities. Although readily phosphorylated by CheA[260-537], the CheA catalytic domain, CheA[1-149], was a poor substrate for transphosphorylation by full-length CheA molecules, implying that the resident P1 domain monopolizes the CheA catalytic center. CheA-H48Q, a nonphosphorylatable mutant, failed to transphosphorylate CheA[1-149], suggesting that phosphorylation of the P1 domain in cis may alleviate the exclusion effect. In agreement with these findings, a 40-fold excess of CheA[1...Continue Reading

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Citations

Jan 23, 1998·Nature Structural Biology·M WelchJ P Samama
Oct 19, 2005·Proceedings of the National Academy of Sciences of the United States of America·Claudia A Studdert, John S Parkinson
Apr 29, 2020·Journal of Bacteriology·Se-Young JunGerald L Hazelbauer
Dec 22, 2005·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Melinda D BakerJeffry B Stock
Sep 11, 2019·Journal of Bacteriology·Germán E Piñas, John S Parkinson
Mar 31, 2018·Frontiers in Cellular and Infection Microbiology·Haritha AdhikarlaAlbert I Ko
Jun 9, 2010·Molecular Microbiology·Kathryn A ScottJudith P Armitage
Apr 21, 2017·Protein Science : a Publication of the Protein Society·Wenlin PanGerald L Hazelbauer
Feb 28, 2018·Journal of Bacteriology·Xueye DingXiqing Wang
Mar 20, 2003·Journal of Bacteriology·Brian J CantwellMichael D Manson
Mar 13, 2001·Journal of Molecular Biology·C KimS Khan
Oct 26, 2005·Biochemistry·Run-Zhi LaiMichael D Manson
Aug 18, 2005·Journal of the American Chemical Society·Jian ZhangHualiang Jiang

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