PMID: 7544817Aug 18, 1995Paper

Chemotaxis of T lymphocytes on extracellular matrix proteins. Analysis of the in vitro method to quantitate chemotaxis of human T cells

Journal of Immunological Methods
D D TaubS M Turcovski-Corrales

Abstract

The present report compares a variety of T cell purification protocols and chemotaxis procedures in assessing chemokine-induced T cell migration using a microchemotaxis assay. Rapidly purified T cells are capable of directly responding to the beta chemokines macrophage inflammatory protein-1 alpha (MIP-1 alpha), MIP-1 beta, and RANTES in the absence of alpha CD3 stimulation as previously described (Taub, D.D. and Oppenheim, J.J. (1993) Cytokine 5, 175). However, T cell purification schemes involving prolonged 37 degrees C incubations generally produce non-motile T lymphocytes that require stimulation with alpha CD3 antibody for 6-12 h in culture to recover chemotactic mobility. This loss of chemotactic potential appears to be due to prolonged 37 degrees C incubations as rapidly purified T cells lose migratory activity upon incubation at 37 degrees C. Radiolabeled binding analysis revealed that beta chemokine binding sites are downregulated as short as 2 h after incubation at 37 degrees C. T cells require the presence of extracellular matrix molecules to facilitate T cell migration. While many of these proteins permit chemotactic activity, human plasma and foreskin fibronectin were found to be the most effective matrix molecule ...Continue Reading

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Citations

Mar 4, 1998·European Journal of Immunology·S J RothT A Springer
Jul 1, 2005·Journal of Immunological Methods·Reena P VishwanathMichael C Jensen
Dec 1, 1999·Developmental and Comparative Immunology·K M Lam
Dec 1, 1996·Cytokine & Growth Factor Reviews·D D Taub
Jan 11, 2005·The Journal of Burn Care & Rehabilitation·Ankush Gosain, Richard L Gamelli
Jul 15, 2009·American Journal of Respiratory Cell and Molecular Biology·Denise Al AlamSophie Catherine Gangloff
Feb 28, 2002·Proceedings of the National Academy of Sciences of the United States of America·Fabrice AllainGeneviève Spik
Aug 12, 2009·European Journal of Immunology·Meiyue ChenJian Hong
Jun 1, 1997·European Journal of Immunology·I UtsunomiyaJ M Wang
Dec 18, 1998·The Journal of Investigative Dermatology·R KunstfeldP Petzelbauer
Apr 25, 2008·Current Protocols in Immunology·Dennis D Taub, Eric Schaffer
Jun 23, 1998·The Journal of Biological Chemistry·J HesselgesserR Horuk
Feb 7, 1998·The Journal of Biological Chemistry·R HorukR W Doms
Feb 13, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·S TillI Sabroe
Apr 24, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Tim R TraynorGary B Huffnagle
Nov 22, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·J A MacLeanA D Luster
Aug 18, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·S FranitzaO Lider

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