PMID: 602940Jan 1, 1977Paper

Chemotaxis radioassays: a simplified, quantitative method using technetium-99m radiocolloid labeled granulocytes

American Journal of Hematology
D English, T A McPherson

Abstract

Selective labeling of phagocytes in human blood can be achieved by incubation of unseparated, heparinized blood with 99mTc-sulfur colloid. We have developed a method for quantitation of granulocyte chemotaxis using granulocytes labeled by this technique. The method was found to be more accurate and less tedious to perform than the conventional Boyden chamber chemotaxis assay. The assay was also less time-consuming and as quantitative as a previously described granulocyte chemotaxis radioassay in which 51Cr-labeled leukocytes were used. The simplicity and reliability of this technique and the ready availability of 99mTc-sulfur colloid may provide one standardized method for granulocyte chemotaxis quantitation in clinical and experimental medicine.

References

Jul 1, 1976·The Journal of Clinical Investigation·H Rosen, S J Klebanoff
Oct 1, 1976·The Journal of Clinical Investigation·B D ChesonB M Babior
Sep 7, 1974·Lancet·N D CharkesR Maguire
Apr 6, 1972·The New England Journal of Medicine·A N DeMeo, B R Andersen
Aug 1, 1966·The American Journal of Roentgenology, Radium Therapy, and Nuclear Medicine·D D PattonM M Webber

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Citations

Jan 1, 1978·International Journal of Nuclear Medicine and Biology·T D HoranA A Noujaim
Jul 1, 1979·Journal of Cellular Physiology·L A BoxerR L Baehner

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