Chemotaxis to atrazine and detection of a xenobiotic catabolic plasmid in Arthrobacter sp. DNS10.

Environmental Science and Pollution Research International
Ying ZhangXiaonan Dong

Abstract

A plasmid named pDNS10 was detected from an atrazine-degrading strain Arthrobacter sp. DNS10 which has been isolated previously in our laboratory. In this paper, a special plasmid-detecting method and drop assays experiments were mainly used to achieve research goals. pDNS10 exhibited an excellent stability because it also could be detected even when the strain DNS10 has been subcultured under nonselective conditions for eight times. Over a 48-h incubation period, the OD(600) of samples inoculated with strain DNS10 and strain DNS10-ST (both of them contained pDNS10) were 0.31 ± 0.042 and 0.305 ± 0.034, respectively ,whereas the OD(600) of samples inoculated strain without pDNS10 (strain DNS10-PE) was only 0.138 ± 0.018. No atrazine was detected in the inoculated strain DNS10 and strain DNS10-ST samples at this period. Contrarily, the atrazine-degrading rate of strain DNS10-PE was only 5.23 ± 0.71%. Furthermore, both the two types of strains containing pDNS10 confirmed the presence of known degrading genes such as trzN, atzB, and atzC. It suggests that pDNS10 is an atrazine catabolic plasmid. In drop assays experiments, the wild-type strain DNS10 cells were chemotactically attracted to atrazine, whereas strain DNS10-PE showed no...Continue Reading

References

Mar 1, 1981·Journal of Bacteriology·C I Kado, S T Liu
Apr 16, 1998·Journal of Bacteriology·M L de SouzaL P Wackett
Mar 4, 2000·Canadian Journal of Microbiology·S K Samanta, R K Jain
Jun 13, 2001·Journal of Environmental Quality·J E BarbashR J Gilliom
Feb 8, 2002·Applied Microbiology and Biotechnology·L P WackettN Shapir
Apr 18, 2002·Proceedings of the National Academy of Sciences of the United States of America·Tyrone B HayesAaron Vonk
Jun 12, 2002·Current Opinion in Microbiology·Rebecca E Parales, Caroline S Harwood
Nov 27, 2002·Applied and Environmental Microbiology·Gunjan Pandey, Rakesh K Jain
Nov 27, 2002·Applied and Environmental Microbiology·Lisa C StrongLawrence P Wackett
Jul 9, 2004·Applied and Environmental Microbiology·James K FredricksonFred J Brockman
Sep 3, 2005·Nature Reviews. Microbiology·J Peter Gogarten, Jeffrey P Townsend
Dec 7, 2005·FEMS Microbiology Ecology·Daniel SmithDavid E Crowley
Apr 1, 1995·Applied and Environmental Microbiology·R T MandelbaumL P Wackett
Jul 8, 2009·Applied and Environmental Microbiology·Xianxian Liu, Rebecca E Parales
Jul 1, 2002·FEMS Microbiology Ecology·Sandrine RousseauxAlain Hartmann
Aug 6, 2010·Environmental Geochemistry and Health·Jinhua WangRusong Zhao

❮ Previous
Next ❯

Citations

Feb 2, 2019·Environmental Science and Pollution Research International·Xiaohong GuoYan Wang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bioremediation (ASM)

Bioremediation is the treatment and removal of harmful pollutants or contaminants through the use of microorganisms. Discover the latest research here.