PMID: 9633899Jun 20, 1998Paper

Chemotherapeutic drug-induced apoptosis in human leukaemic cells is independent of the Fas (APO-1/CD95) receptor/ligand system

British Journal of Haematology
A J McGahonT G Cotter

Abstract

The potential role of the Fas (CD95/APO-1) receptor/ligand system in chemotherapeutic drug-induced apoptosis was examined in a number of human leukaemic cell lines. Flow cytometric profiles of doxorubicin-treated HL-60, K562, U937 and Jurkat cells failed to show any significant increase in Fas or Fas ligand expression over 24 h, despite the induction of significant levels of apoptosis in these cells. Although preincubation of human leukaemic cells with a neutralizing anti-Fas IgG antibody blocked anti-Fas IgM-induced apoptosis, this strategy failed to inhibit chemotherapeutic drug-induced apoptosis. To determine whether recruitment of the Fas/Fas ligand complex during drug-induced apoptosis was a cell-specific event we utilized the CEM cell line. Doxorubicin treatment of CEM cells over 24 h failed to show any up-regulation in Fas or Fas ligand protein levels as detected by flow cytometry. Furthermore, neutralizing anti-Fas IgG Ab failed to inhibit chemotherapeutic drug-induced apoptosis in CEM cells. The present studies do, however, demonstrate a role for anti-Fas IgM Ab in producing a cytotoxic synergistic effect when used in combination with chemotherapeutic drugs. Low-dose anti-Fas IgM treatment in combination with doxorubic...Continue Reading

References

Jan 1, 1992·Annual Review of Immunology·J J CohenK S Sellins
Feb 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·D Wolf, V Rotter
Jan 1, 1980·International Review of Cytology·A H WyllieA R Currie
Jul 1, 1995·Leukemia Research·H D Preisler
Aug 30, 1996·The Journal of Biological Chemistry·A KrippnerB M Babior
Oct 31, 1996·Experientia·B ZhivotovskyS Orrenius
Jan 1, 1996·Ultrasound in Obstetrics & Gynecology : the Official Journal of the International Society of Ultrasound in Obstetrics and Gynecology·L MooreD Chitayat
Jan 1, 1995·Trends in Cell Biology·C D BortnerJ A Cidlowski

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Citations

Jan 24, 2003·Journal of Tongji Medical University = Tong Ji Yi Ke Da Xue Xue Bao·P ZouJ Xiao
Jan 4, 2001·Leukemia Research·K V CullenM W Davey
Mar 29, 2002·British Journal of Haematology·Helmut R SalihVolkmar Nuessler
May 15, 2004·Thyroid : Official Journal of the American Thyroid Association·C MassartJ Gibassier
Jan 30, 2002·Antioxidants & Redox Signaling· Bhat KPLJ M Pezzuto
Apr 26, 2006·The Journal of School Health·Betty T IzumiMichael W Hamm
Aug 20, 2010·Journal of Obesity·Meghan E McGradyElizabeth Holtzapple
Mar 8, 2006·BMC Cancer·Christine L ChafferElizabeth D Williams
Dec 25, 2003·Leukemia & Lymphoma·Kourosh LotfiFreidoun Albertioni
Feb 3, 2005·International Journal of Cancer. Journal International Du Cancer·Min-Young ParkEunhee Kim
Jan 3, 2013·The Journal of Pharmacy and Pharmacology·Oktay TacarCrispin R Dass
Apr 6, 2001·The American Journal of Pathology·I Meinhold-HeerleinJ C Reed
Mar 31, 2000·Experimental Cell Research·S H Kaufmann, W C Earnshaw
Oct 25, 2001·Blood·I Herr, K M Debatin
Mar 13, 1999·The Journal of Biological Chemistry·O MicheauM T Dimanche-Boitrel
Mar 13, 2001·Leukemia·D M O'Gorman, T G Cotter
Mar 15, 2000·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·J FellenbergK M Debatin
Jul 17, 1999·British Journal of Cancer·A P Costa-Pereira, T G Cotter
May 22, 2018·Journal of the Egyptian National Cancer Institute·Fatma AshourMohamed Kamal
Jun 18, 2002·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Marie-Christine EtienneGérard Milano
Jul 27, 2000·Annals of the New York Academy of Sciences·J O'ConnellF Shanahan
Jun 22, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·Y Lee, E Shacter

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis