PMID: 9181462May 1, 1997Paper

Chemotherapeutic purine analogs alter the level of interferon-beta mRNA induced by poly I-poly C in cultured osteosarcoma cells

Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research
D J SlomianyR A Petryshyn

Abstract

The purine nucleoside analogs fludarabine, 2-chlorodeoxyadenosine, and 2'-deoxycoformycin exhibit impressive activity in lymphoproliferative malignancies of adults and children. Their mechanism of action is not clear. Studies have suggested that their use is associated with significant myelosuppression, immunosuppression, and in some circumstances, increased infection with viral and opportunistic pathogens. Because interferons (IFNs) are known to have immunomodulatory activity as well as potent antiproliferative and antiviral activity, we examined whether the chemotherapeutic purine nucleoside analogs alter interferon-beta (IFN-B) gene expression in MG63 in human osteosarcoma cells. Northern blot analysis showed a dose-dependent inhibition of IFN-B mRNA accumulation in response to a known inducer (Poly I-Poly C) all three purine analogs. Hybridization analysis also revealed that inhibition of IFN-beta mRNA accumulation by the purine analogs is not a result of decreased mRNA stability. Further analysis of gene expression by PCR differential display indicated that the effect of the purine analogs was restricted to only a limited number of inducible genes. The data suggest that these molecules alter the signaling process involved ...Continue Reading

References

Jun 11, 1992·Nucleic Acids Research·L DaigneaultD Skup
May 1, 1991·Journal of Cellular Biochemistry·D S HainesD H Gillespie
Mar 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·R PetryshynI M London
Feb 1, 1985·The Journal of Clinical Investigation·S SetoD A Carson
Jun 1, 1986·Clinical Immunology and Immunopathology·C MessinaC Lopez
Mar 1, 1986·Molecular and Cellular Biology·T EnochT Maniatis
Jan 1, 1983·Methods in Enzymology·R PetryshynI M London
Oct 19, 1995·Nature·J N Ihle
Aug 1, 1995·Journal of Pediatric Hematology/oncology·R J Wells, C A Arndt
Sep 1, 1995·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·B D Cheson
Jul 5, 1994·Proceedings of the National Academy of Sciences of the United States of America·A KumarB R Williams
May 1, 1993·Annals of Oncology : Official Journal of the European Society for Medical Oncology·L BergmannD Hoelzer
Jan 1, 1996·Cancer Investigation·D V Kalvakolanu, E C Borden

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antivirals (ASM)

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Antivirals

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.