Chemotherapy-induced oral mucositis and associated infections in a novel organotypic model

Molecular Oral Microbiology
T SobueA Dongari-Bagtzoglou

Abstract

Oral mucositis is a common side effect of cancer chemotherapy, with significant adverse impact on the delivery of anti-neoplastic treatment. There is a lack of consensus regarding the role of oral commensal microorganisms in the initiation or progression of mucositis because relevant experimental models are non-existent. The goal of this study was to develop an in vitro mucosal injury model that mimics chemotherapy-induced mucositis, where the effect of oral commensals can be studied. A novel organotypic model of chemotherapy-induced mucositis was developed based on a human oral epithelial cell line and a fibroblast-embedded collagen matrix. Treatment of organotypic constructs with 5-fluorouracil (5-FU) reproduced major histopathologic characteristics of oral mucositis, such as DNA synthesis inhibition, apoptosis and cytoplasmic vacuolation, without compromising the three-dimensional structure of the multilayer organotypic mucosa. Although structural integrity of the model was preserved, 5-FU treatment resulted in a widening of epithelial intercellular spaces, characterized by E-cadherin dissolution from adherens junctions. In a neutrophil transmigration assay we discovered that this treatment facilitated transport of neutrophi...Continue Reading

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Citations

Apr 23, 2019·PLoS Pathogens·Martinna BertoliniAnna Dongari-Bagtzoglou
Nov 8, 2018·Journal of Fungi·Daniel Montelongo-Jauregui, Jose L Lopez-Ribot
Mar 7, 2020·Tissue Engineering. Part B, Reviews·Fahimeh TabatabaeiLobat Tayebi
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Feb 2, 2021·FEMS Microbiology Reviews·Antonia LastBernhard Hube
Nov 5, 2019·Bio-protocol·Takanori SobueAnna Dongari-Bagtzoglou
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Sep 19, 2020·Archives of Oral Biology·Vanessa Castro de Souza E SilvaEvandro Watanabe
Nov 4, 2019··Martinna BertoliniAnna Dongari-Bagtzoglou

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