Chestnut bur-shaped aggregates of chrysotile particles enable inoculation of Escherichia coli cells with plasmid DNA

Applied Microbiology and Biotechnology
N Yoshida, Y Saeki

Abstract

In the present study, Escherichia coli cells exhibited antibiotic resistance after transformation with exogenous plasmid DNA adsorbed onto chrysotile particles during agar-exposure. We previously demonstrated penetration of E. coli by chrysotile particles during agar-exposure. To further investigate the mechanism by which transformation of E. coli is achieved through the use of chrysotile fibers, the interaction between E. coli cells and chrysotile was examined during agar-exposure. Dispersion of chrysotile particles within the chrysotile solution was analyzed by flow cytometry. A suspension containing E. coli cells expressing blue fluorescence protein and chrysotile particles was exposed to agar using stirring apparatus, which allowed a constant vertical reaction force to be applied to the surface of the gel. Fluorescence microscopy was then used to illustrate the adsorption of fluorescein isothiocyanate-conjugated DNA oligomers to chrysotile. Larger aggregates were observed when increasing concentrations of chrysotile were added to the solution. With prolonged exposure, during which surface moisture diffused into the agar gel, greater concentrations of chrysotile were observed on the agar surface. In addition, chrysotile aggr...Continue Reading

Citations

May 28, 2009·Applied Microbiology and Biotechnology·Naoto Yoshida, Misa Sato
Apr 26, 2006·Journal of Industrial Microbiology & Biotechnology·Naoto Yoshida, Kazuki Takebe
Apr 17, 2009·Astrobiology·Naoto Yoshida, Nori Fujiura
Jan 1, 2010·International Journal of Molecular Sciences·Haidong TanMasaharu Seno
Dec 17, 2009·Journal of Microbiological Methods·Gottfried WilharmEvelyn Skiebe
May 15, 2015·Journal of Microbiological Methods·G P MendesM Mota
Jun 9, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Michele BecceMolly M Stevens

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