CheW binding interactions with CheA and Tar. Importance for chemotaxis signaling in Escherichia coli

The Journal of Biological Chemistry
Marina BoukhvalovaRichard C Stewart

Abstract

The initial signaling events underlying the chemotactic response of Escherichia coli to aspartic acid occur within a ternary complex that includes Tar (an aspartate receptor), CheA (a protein kinase), and CheW. Because CheW can bind to CheA and to Tar, it is thought to serve as an adapter protein in this complex. The functional importance of CheW binding interactions, however, has not been investigated. To better define the role of CheW and its binding interactions, we performed biochemical characterization of six mutant variants of CheW. We examined the ability of the purified mutant CheW proteins to bind to CheA and Tar, to promote formation of active ternary complexes, and to support chemotaxis in vivo. Our results indicate that mutations which eliminate CheW binding to Tar (V36M) or to CheA (G57D) result in a complete inability to form active ternary complexes in vitro and render the CheW protein incapable of mediating chemotaxis in vivo. The in vivo signaling pathway can, however, tolerate moderate changes in CheW-Tar and CheW-CheA affinities observed with several of the mutants (G133E, G41D, and 154ocr). One mutant (R62H) provided surprising results that may indicate a role for CheW in addition to binding CheA/receptors a...Continue Reading

References

Aug 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·J B Stock, D E Koshland
Feb 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·J A Gegner, F W Dahlquist
Jul 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·D F McNally, P Matsumura
Jan 1, 1990·Methods in Enzymology·F W StudierJ W Dubendorff
Dec 21, 1990·Cell·K A Borkovich, M I Simon
Nov 1, 1989·Analytical Biochemistry·S C Gill, P H von Hippel
Feb 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·K A BorkovichM I Simon
Sep 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·A J Wolfe, H C Berg
Nov 1, 1989·Journal of Bacteriology·D A SandersD E Koshland
Nov 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J D Liu, J S Parkinson
Jan 1, 1988·Methods in Enzymology·J G Nørby
Jan 1, 1988·Cold Spring Harbor Symposia on Quantitative Biology·A M StockJ B Stock
Mar 15, 1988·Biochemical and Biophysical Research Communications·D WylieJ Stock
Nov 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·J F HessM I Simon
Jan 1, 1974·Methods in Enzymology·M J Osborn, R Munson
Aug 12, 1966·Science·J Adler
Jan 1, 1967·Journal of Bacteriology·J B ArmstrongM M Dahl
Sep 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·R A Smith, J S Parkinson
Oct 1, 1994·Journal of Bacteriology·P Ames, J S Parkinson
Oct 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·M WelchM Eisenbach
Feb 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·A J Wolfe, R C Stewart
Jun 26, 1997·Nature·N Barkai, S Leibler
Mar 12, 1998·Journal of Bacteriology·M D MansonR M Macnab
Apr 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·T M YiJ Doyle
Jan 19, 2002·Nature Structural Biology·Ian J GriswoldFrederick W Dahlquist
Jan 1, 1953·Advances in Protein Chemistry·G WEBER

❮ Previous
Next ❯

Citations

Apr 2, 2005·Current Opinion in Microbiology·John S ParkinsonClaudia A Studdert
Apr 20, 2006·Nature Structural & Molecular Biology·Sang-Youn ParkBrian R Crane
Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Ariane BriegelGrant J Jensen
May 25, 2011·Proceedings of the National Academy of Sciences of the United States of America·Mingshan Li, Gerald L Hazelbauer
May 19, 2006·Journal of Bacteriology·Stuart WrightShahid Khan
Sep 22, 2009·Journal of Bacteriology·Manuel MoisiStefan Schild
Jan 22, 2008·Journal of Bacteriology·Robert BelasZhaomin Yang
Jun 10, 2004·Microbiology and Molecular Biology Reviews : MMBR·Hendrik Szurmant, George W Ordal
Nov 23, 2012·BMC Microbiology·Matthias SchlesnerDieter Oesterhelt
Jan 13, 2010·BMC Systems Biology·Rebecca HamerCharlotte M Deane
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Peijun ZhangSriram Subramaniam
Oct 19, 2005·Proceedings of the National Academy of Sciences of the United States of America·Claudia A Studdert, John S Parkinson
Dec 14, 2011·Journal of Molecular Biology·Anh VuFrederick W Dahlquist
Dec 28, 2010·Journal of Molecular Biology·Tae Seok MoonChristopher A Voigt
Sep 14, 2010·Trends in Microbiology·Roger P AlexanderKaren M Ottemann
Jul 17, 2007·Biochemical and Biophysical Research Communications·You LiChangwen Jin
Mar 28, 2008·Molecular Microbiology·Ariane BriegelGrant J Jensen
Apr 1, 2006·Molecular Microbiology·Susan M ButlerAndrew Camilli
May 25, 2011·Environmental Microbiology·Jesús LacalJuan L Ramos
Oct 14, 2011·Molecular Microbiology·Ariane BriegelGrant J Jensen
Dec 23, 2009·Molecular Microbiology·Lauren E Hartley-TassellVictoria Korolik
May 22, 2010·Molecular Microbiology·Marcos J CardozoClaudia A Studdert
Nov 13, 2004·Trends in Microbiology·Victor Sourjik
Mar 1, 2012·European Journal of Microbiology & Immunology·A E ZautnerR Lugert
Nov 7, 2014·Cellular and Molecular Life Sciences : CMLS·Shuangyu Bi, Luhua Lai
Jul 31, 2004·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·Renate Lux, Wenyuan Shi
Nov 29, 2017·Molecular Plant-microbe Interactions : MPMI·Zhiwei HuangMinliang Guo
Feb 21, 2018·Biomedit︠s︡inskai︠a︡ khimii︠a︡·D V GrishinN N Sokolov
Apr 20, 2002·The Journal of Biological Chemistry·Marina BoukhvalovaRichard C Stewart

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.