CHFR promotes the migration of human gastric cancer cells by inducing epithelial-to-mesenchymal transition in a HDAC1-dependent manner

OncoTargets and Therapy
Shangwen YangBin Ye

Abstract

Previous studies have illustrated that checkpoint with forkhead-associated and ring finger domains (CHFR) was frequently silenced in several cancer types due to promoter hypermethylation and functions as a tumor suppressor gene. However, the data from the public dataset reveal that CHFR is highly expressed in human gastric cancer specimens, and the biological function of CHFR in gastric cancer is still not well understood. The clinical association between CHFR expression and the overall survival of gastric cancer patients as well as cancer metastasis was analyzed according to public datasets. The CHFR expression in clinical specimens and human gastric cancer cell lines was detected by immunohistochemistry and Western blotting, respectively. Gain (overexpression) and loss (silencing) of function experiments were used to elucidate the role of CHFR in gastric cancer. The migration ability of gastric cancer cells was determined by wound healing and transwell assays. Cell cycle distribution was analyzed using fluorescence-activated cell sorting experiment. The expression of the proteins in cancer cells was measured using Western blot analysis. According to the analysis from Kaplan-Meier plotter dataset, CHFR expression was negativel...Continue Reading

Citations

Jan 5, 2021·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Huizhen WangYongxiang Li
Jan 16, 2021·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Sabateeshan MathavarajahRobert J Huber
Feb 12, 2021·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Naila ShoaibNousheen Zaidi
Apr 18, 2021·Biochemical and Biophysical Research Communications·Ying XuQianjun Li
Jul 10, 2021·Frontiers in Cell and Developmental Biology·Yue WuTao Wang

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Methods Mentioned

BETA
biopsy
electrophoresis
transfection
flow cytometry
fluorescence-activated cell sorting
ubiquitination

Software Mentioned

GraphPad
GraphPad Prism

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