Chibby suppresses aerobic glycolysis and proliferation of nasopharyngeal carcinoma via the Wnt/β-catenin-Lin28/let7-PDK1 cascade.

Journal of Experimental & Clinical Cancer Research : CR
Cheng-Fu CaiShu-Yu Yang

Abstract

Great progress has been achieved in the study of the aerobic glycolysis or the so-called Warburg effect in a variety of cancers; however, the regulation of the Warburg effect in Nasopharyngeal carcinoma (NPC) has not been completely defined. Gene expression pattern of NPC cells were used to test associations between Chibby and β-catenin expression. Chibby siRNAs and over-expression vector were transfected into NPC cells to down-regulate or up-regulate Chibby expression. Loss- and gain-of function assays were performed to investigate the role of Chibby in NPC cells. Western blot, cell proliferation, Glucose uptake, Lactate release, ATP level, and O2 consumption assays were used to determine the mechanism of Chibby regulation of underlying targets. Finally, immunohistochemistry assay of fresh NPC and nasopharyngeal normal tissue sample were used to detect the expression of Chibby, β-Catenin, and PDK1 by immunostaining. We observed that Chibby, a β-catenin-associated antagonist, is down-regulated in nasopharyngeal carcinoma cell lines and inhibits Wnt/β-Catenin signaling induced Warburg effect. Mechanism study revealed that Chibby regulates aerobic glycolysis in NPC cells through pyruvate dehydrogenase kinase 1(PDK1), an important...Continue Reading

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Citations

Aug 20, 2018·International Journal of Cancer. Journal International Du Cancer·Tereza GoliasIoanna Papandreou
May 11, 2020·Virchows Archiv : an International Journal of Pathology·Jinfeng ZhuXiaolin Wang
Aug 28, 2020·World Journal of Stem Cells·Zi-Jian ZhangYu Wen
Jul 3, 2019·Cancers·Sara El-SahliSheng Liu
Jul 31, 2019·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·Z WuJ Jin
Jul 7, 2020·Frontiers in Oncology·Eduardo Silva-Pavez, Julio C Tapia
Dec 15, 2020·Critical Reviews in Oncology/hematology·Chiman MohammadiRezvan Najafi
May 24, 2021·Biochimica Et Biophysica Acta. Reviews on Cancer·Saleha AnwarMd Imtaiyaz Hassan
May 6, 2021·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Yu-Ying YangJian-Min Liu
Aug 17, 2021·Frontiers in Cell and Developmental Biology·Yuqiang LiCenap Güngör

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Methods Mentioned

BETA
nuclear translocation
xenograft
xenografts

Software Mentioned

Chibby

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