PMID: 8963959Jan 1, 1996Paper

Chick embryo liver microsomal steroid hydroxylations: induction by dexamethasone, phenobarbital, and glutethimide and inactivation following the in ovo administration of porphyrinogenic compounds

Canadian Journal of Physiology and Pharmacology
S M KimmettG S Marks

Abstract

Metabolites of [4-(14)C]androstenedione (AD) and [4-(14)C]progesterone (PG) were separated and quantitated following incubation with hepatic microsomes from chick embryos. PG 2 alpha-hydroxylase and AD 7 alpha-hydroxylase, which are diagnostic markers in rat liver for cytochrome P450 (P450) 2C11 and 2A1/2, respectively, were not identified in chick embryo liver. PG 6 beta-hydroxylase and AD 6 beta-hydroxylase, diagnostic markers for P450 3A1/2 activity in rat liver, were identified in chick embryo liver, and we were able to show that radiolabeled 6 beta-hydroxyprogesterone and 6 beta-hydroxyandrostenedione, the metabolites of PG and AD 6 beta-hydroxylase, respectively, were homogeneous and identical with authentic standards. Dexamethasone, phenobarbital, and glutethimide were found to be significant inducers of PG and AD 6 beta-hydroxylases in chick embryo liver. The in ovo administration of the porphyrinogenic compounds 3,5-diethoxycarbonyl-1,4-dihydro-2,6-dimethyl-4-ethylpyridine (4-ethyl DDC) and 3-[2-(2,4,6-trimethylphenyl)-thioethyl]-4-methylsydnone (TTMS) caused inactivation of chick embryo hepatic PG and AD 6 beta-hydroxylases. Therefore, we suggest that PG and AD 6 beta-hydroxylases may serve as diagnostic markers for a...Continue Reading

References

Oct 1, 1991·Archives of Biochemistry and Biophysics·D J WaxmanK Korzekwa
Feb 15, 1990·Archives of Biochemistry and Biophysics·M HalvorsonA Parkinson
Oct 1, 1990·Archives of Biochemistry and Biophysics·R P GuptaM B Abou-Donia
Jun 1, 1990·Biochemical Pharmacology·J E MackieG S Marks
Jan 1, 1990·Pharmacology & Therapeutics·D E Ryan, W Levin
Jan 1, 1989·Drug Metabolism Reviews·M A CorreiaK Q Yao

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Citations

Sep 28, 2001·Journal of Dairy Science·S M MacielL J Spicer

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