PMID: 698824Oct 6, 1978Paper

Chick sympathetic neurons develop receptors for alpha-bungarotoxin in vitro, but the toxin does not block nicotinic receptors

Brain Research
E D KouvelasL A Greene

Abstract

Studies were carried out on the development and physiological role of receptors for alpha-bungarotoxin (alphaBT) on chick embryo sympathetic neurons maintained in dissociated cell culture. Neurons from embryos of 13 days incubation (E13) developed alphaBT receptors in vitro with a time course and to a maximum level per cell similar to that previously observed for such neurons in vivo. In vitro receptor development by E11 and E8 neurons was also present, but (in comparison with E13 neurons) reached somewhat lower maximal levels. Receptor development in vitro was not affected by exclusion of non-neuronal cells from the cultures. In the present and in previous studies, binding of alphaBT to chick sympathetic neurons was blocked by a variety of ligands of nicotinic acetylcholine receptors. However, saturating concentrations of toxin were found here to be ineffective in blocking either (a) release of [3H]norepinephrine from the cultured neurons elicited via nicotinic stimulation of acetylcholine receptors or (b) depolarizing responses of the cultured neurons elicited by iontophoretically applied acetylcholine and nicotine. Kinetic studies further revealed that, while the idssociation of alphaBT from the cultured neurons is considera...Continue Reading

References

Nov 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·E J FurshpanD D Potter
Jun 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·Z Vogel, M Nirenberg
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Citations

Aug 18, 1980·Life Sciences·R E Oswald, J A Freeman
Jan 1, 1982·Pharmacology & Therapeutics·A T EldefrawiM E Eldefrawi
Feb 1, 1988·Trends in Neurosciences·R H Loring, R E Zigmond
Apr 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·P M Ravdin, D K Berg
Mar 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·L M Marshall
Feb 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·R I NormanJ O Dolly
Nov 25, 1998·Proceedings of the National Academy of Sciences of the United States of America·M G LópezA G García
Jul 1, 1984·Journal of Neurochemistry·M R Whipple, D G Drescher
Dec 1, 1981·Journal of Neurochemistry·R E Oswald, J A Freeman
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Jan 1, 1987·Molecular Neurobiology·J LindstromP Whiting
Jun 1, 1981·British Journal of Pharmacology·B CollierP A Smith
Jun 1, 1981·European Journal of Biochemistry·H Betz

Related Concepts

Acetylcholine Sulfate (1: 1)
Adrenergic Fibers
kappa-Bungarotoxin
Chick Embryo
Autonomic Ganglion
Resting Potentials
Neurons
Norepinephrine, (+, -)-Isomer
Cholinergic Receptors
Nicotinic Receptors

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