Chick tendon fibroblast transcriptome and shape depend on whether the cell has made its own collagen matrix

Scientific Reports
Ching-Yan Chloé YeungKarl E Kadler

Abstract

Collagen- and fibrin-based gels are extensively used to study cell behaviour. However, 2D-3D and collagen-fibrin comparisons of gene expression, cell shape and mechanotransduction, with an in vivo reference, have not been reported. Here we compared chick tendon fibroblasts (CTFs) at three stages of embryonic development with CTFs cultured in collagen- or fibrin-based tissue engineered constructs (TECs). CTFs synthesised their own collagen matrix in fibrin-based TECs and better recapitulated the gene expression, collagen fibril alignment and cell shape seen in vivo. In contrast, cells in 3D collagen gels exhibited a 2D-like morphology and expressed fewer of the genes expressed in vivo. Analysis of YAP/TAZ target genes showed that collagen gels desensitise mechanotransduction pathways. In conclusion, gene expression and cell shape are similar on plastic and 3D collagen whereas cells in 3D fibrin have a shape and transcriptome better resembling the in vivo situation. Implications for wound healing are discussed.

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Citations

Oct 12, 2016·Scientific Reports·Michael J RandlesRachel Lennon
Jan 8, 2020·Nature Cell Biology·Joan ChangKarl E Kadler
Feb 26, 2020·BMC Biomedical Engineering·Sophia K Theodossiou, Nathan R Schiele
Mar 20, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Adam PickardKarl E Kadler
Apr 16, 2021·Journal of the Mechanical Behavior of Biomedical Materials·A GiannopoulosS P Magnusson
Aug 22, 2020·Journal of Proteome Research·Cheng ZhangChing-Yan Chloé Yeung
Oct 3, 2021·Npj Regenerative Medicine·Paula Sarmiento, Dianne Little

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Datasets Mentioned

BETA
E-MTAB-3488

Methods Mentioned

BETA
dissection
electrophoresis
PCA
electron microscopy
transmission electron microscopy

Software Mentioned

Limma
lmFit
Ingenuity Pathways
maxdView
IPA
QVALUE
DAVID
Venny
eBayes
Database for Annotation , Visualisation and Integrated Discove...

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