Chicken egg yolk antibodies (IgY) for detecting circulating antigens of Schistosoma japonicum

Parasitology International
Yu-Chun CaiJia-Xu Chen

Abstract

IgY isolated from egg yolk has been widely used in immunodiagnostic tests, including tests to detect circulating antigen (soluble egg antigen or SEA) of Schistosoma japonicum. A sandwich ELISA was established using a combination of anti-S. japonicum SEA-IgY polyclonal antibodies and IgM monoclonal antibodies. To explore sensitivity and specificity of the sandwich ELISA, serum samples from 43 patients infected with S. japonicum were tested. All acute cases and 91.3% of the chronic cases showed a positive reaction. Only 5% of the control sera from healthy persons gave a positive response. Cross-reactions with antibodies to nine other parasites were rare. The developed immunoassay is reasonably sensitive and specific. It could be used for field research and treatment efficacy assessments.

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Citations

Jun 12, 2014·Veterinary Parasitology·Luzia Cristina Lencioni SampaioSilvia Gonzalez Monteiro
Aug 1, 2015·Clinical Microbiology Reviews·Kosala G A D WeerakoonDonald P McManus
Apr 13, 2017·Preparative Biochemistry & Biotechnology·Cui LiXiaoying Zhang
Sep 10, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ulrike DapuntJan Philippe Kretzer

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