PMID: 2123097Nov 1, 1990Paper

Chicken liver Pz-peptidase, a thiol-dependent metallo-endopeptidase

The Biochemical Journal
A J Barrett, M A Brown

Abstract

Pz-peptidase was purified from chicken liver as a protein of Mr 80,000 and pI 5.2. The purified enzyme hydrolysed phenylazobenzyloxycarbonyl-Pro-Leu-Gly-Pro-D-Arg, 2,4-dinitrophenyl-Pro-Leu-Gly-Pro-Trp-D-Lys. 7-methoxycoumarin-3-carboxylyl-Pro-Leu-Gly-Pro-D-(2,4-dinitropheny l)Lys, benzoyl-Gly-Ala-Ala-Phe-p-aminobenzoate, Ac-Ala4 (at the Ala-1-Ala-2 bond) and bradykinin (at the Phe-5-Ser-6 bond). No hydrolysis of proteins was detected. Loss of activity in the presence of EDTA or 1,10-phenanthroline was time-dependent. Metal ions found to restore activity after treatment with EDTA were Zn2+, Mn2+, Ca2+, Co2+ and Cd2+, in decreasing order of effectiveness. Ni2+, Fe2+ and higher concentrations of Zn2+ were inhibitory. Inhibition by N-[1-(RS)-carboxy-3-phenylpropyl]-Ala-Ala-Tyr-p-aminobenzoate and related compounds showed Ki values (down to 5 nM) somewhat lower than those for the rat enzyme. Pz-peptidase was activated by low concentrations of 2-mercaptoethanol and dithiothreitol, but inhibited by higher concentrations. p-Chloromercuribenzoate and some other thiol-blocking reagents were inhibitory. Inactivation by diethyl pyrocarbonate that was reversible by hydroxylamine showed the presence of essential histidine residue(s). We con...Continue Reading

Citations

Oct 10, 2002·Endocrine Reviews·Corie N ShrimptonRebecca A Lew
Aug 12, 1996·FEBS Letters·A M Hitz, N H Georgopapadakou
Jul 11, 1997·The Journal of Biological Chemistry·C N ShrimptonA I Smith
Jan 15, 1995·European Journal of Biochemistry·R M O'Leary, B O'Connor
Jan 26, 2010·Organic Letters·Mohammad R SeyedsayamdostJon Clardy
Apr 9, 1993·Journal of Chromatography·T ChikumaT Kato
Oct 20, 2005·Journal of Bioscience and Bioengineering·K AkiyamaR Takata
Apr 1, 1996·The International Journal of Biochemistry & Cell Biology·R M O'LearyB O'Connor
Apr 29, 2006·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·V DuboisA Trouet
Nov 3, 2005·Biochemistry·Rory E MortyLuiz Juliano

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