Chicken ovalbumin upstream promoter-transcription factor (COUP-TF) modulates expression of the Purkinje cell protein-2 gene. A potential role for COUP-TF in repressing premature thyroid hormone action in the developing brain.

The Journal of Biological Chemistry
G W AndersonJ H Oppenheimer

Abstract

The cerebellar Purkinje cell-specific PCP-2 gene is transcriptionally activated by thyroid hormone during the 2nd and 3rd weeks of postnatal life in the rat. In contrast, thyroid hormone has no detectable effects on PCP-2 expression in the fetal rat. We now present data that suggest that the orphan nuclear receptor chicken ovalbumin upstream promoter-transcription factor (COUP-TF) represses triiodothyronine (T3)-dependent transcriptional activation of PCP-2 in the immature Purkinje cell. Gel shift assays show that the PCP-2 A1TRE and adjoining sequences (-295/-199 region) bind to rat and mouse brain nucleoproteins in a developmentally regulated fashion and that one of these nucleoproteins could be the orphan nucleoprotein COUP-TF. In support of this hypothesis, in vitro translated COUP-TF binds to the -295/-199 region and COUP-TF represses T3-dependent activation of the PCP-2 promoter in transient transfection analyses. Finally, immunohistochemical studies reveal that COUP-TF is specifically expressed in the immature fetal and early neonatal Purkinje cell and that this expression diminishes coincident with thyroid hormone induction of PCP-2 expression. Our findings are consistent with the hypothesis that the presence or absence...Continue Reading

References

Nov 1, 1989·Endocrine Reviews·M A ShupnikW W Chin
Dec 1, 1986·Molecular and Cellular Biology·I SagamiB W O'Malley
May 1, 1995·Brain Research. Molecular Brain Research·S Lopes da SilvaJ P Burbach
Jun 25, 1995·Nucleic Acids Research·B E Sawaya, E Schaeffer
May 1, 1995·Journal of Neuroscience Research·K NeumanT Neuman
Aug 1, 1997·Endocrinology·H L SchwartzJ H Oppenheimer
Jul 4, 1997·Molecular and Cellular Endocrinology·G W AndersonJ H Oppenheimer

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Citations

Feb 24, 2011·Biological Trace Element Research·Lu ZhangZu Pei Chen
Apr 18, 2008·The Cerebellum·Grant W Anderson
Oct 20, 2009·The Cerebellum·Jacob A Lyons, Joseph R Prohaska
Oct 24, 2000·Brain Research. Molecular Brain Research·A L Dowling, R T Zoeller
Jul 14, 1999·Biochimie·J H Oppenheimer
Feb 7, 2001·Trends in Endocrinology and Metabolism : TEM·N Koibuchi, W W Chin
Aug 30, 2001·Thyroid : Official Journal of the American Thyroid Association·T AsamiM Uchiyama
Dec 4, 2003·Thyroid : Official Journal of the American Thyroid Association·Grant W AndersonSidney A Jones
Sep 5, 2012·The Journal of Clinical Investigation·Gregory A Brent
May 17, 2006·Endocrine Journal·Noriyuki Koibuchi, Toshiharu Iwasaki
Oct 5, 2013·The Cerebellum·Jaroslaw J BarskiMichael Meyer
Apr 9, 2002·Journal of Endocrinological Investigation·J Bernal
Sep 5, 2013·Thyroid : Official Journal of the American Thyroid Association·Antonio C BiancoUNKNOWN American Thyroid Association Task Force on Approaches and Strategies to Investigate Thyroid Hormone Economy and Action
Jun 19, 2007·Best Practice & Research. Clinical Endocrinology & Metabolism·Alexis Oetting, Paul M Yen
Jan 6, 2001·Frontiers in Neuroendocrinology·G W Anderson
Aug 24, 1999·Journal of Neurobiology·A Rodríguez-Peña
Dec 6, 2017·Thyroid : Official Journal of the American Thyroid Association·Xiaochun TengGregory A Brent
Oct 26, 1999·Endocrine Reviews·V Giguère
Jun 28, 2001·Physiological Reviews·P M Yen
Apr 20, 2019·Frontiers in Endocrinology·Barbara K Stepien, Wieland B Huttner

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