Dec 1, 1980

Chicken oxygen dissociation curve by dynamic tonometry

Poultry Science
R L BoydG R McDaniel

Abstract

Oxygen dissociation curves were determined for blood from mature (5 male, 5 female) Single Comb White Leghorn chickens. Blood was oxygenated by bubbling with humidified 95% O2, 5% CO2, 0% N2 at 100 ml/min for 30 min, then gradually deoxygenated with 0% O2, 5% CO2, 95% N2 at 4 ml/min. During deoxygenation .5 ml samples were drawn at 7 in intervals. The PO2, PCO2, and pH were measured polarographically and the content of O2 was measured by gas chromatography. The PO2 was corrected to T = 42 C, pH = 7.5, and PCO2 = 40 Torr. The curves were determined by plotting: 1) PO2 vs. % Saicont based on O2 content of each serial sample as compared to the fully oxygenated blood O2 content, and 2) PO2 vs. duration of dynamic tonometry plots. The curves determined by the two methods of data analysis corresponded closely, the P50 values (x +/- SD) being 36.2 +/- 2.5, 34.3 +/- 3.1 Torr, respectively.

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Mentioned in this Paper

Tonometry
Combid
Leghorn chicken
Comb Animal Structure
Oxygen Consumption
Metazoa
Gas Chromatography
Carbon Dioxide Measurement, Partial Pressure

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