Chicken recombinant antibodies specific for very virulent infectious bursal disease virus

Archives of Virology
S SapatsJ Ignjatovic

Abstract

A phage-displayed single chain variable fragment (scFv) antibody library was constructed from the immune spleen cells of chickens immunized with very virulent infectious bursal disease virus (vvIBDV) strain CS89. A library consisting of around 9.2 x 10(7) clones was subjected to 3 rounds of panning against captured CS89 virus. Analysis of individual clones by nucleotide sequencing revealed at least 22 unique scFv antibodies binding to vvIBDV in ELISA. Testing of the scFv antibody panel in ELISA against classical, variant or vaccine strains and a wide variety of vvIBDV isolates from the UK, China, France, Belgium, Africa, Brazil, Indonesia and the Netherlands identified one antibody, termed chicken recombinant antibody 88 (CRAb 88) that was specific for vvIBDV. CRAb 88 was capable of recognizing all vvIBDV strains tested regardless of their country of origin and showed no reactivity with classical, variant or vaccine strains, lending support to the use of this scFv as a powerful diagnostic tool for the differentiation of vvIBDV strains. Immunoprecipitation studies revealed that CRAb 88 was directed towards a highly conformational epitope located within the major neutralizing protein VP2. Sequence analysis of the hypervariable re...Continue Reading

References

Aug 21, 2002·Journal of Biochemistry, Molecular Biology, and Biophysics : JBMBB : the Official Journal of the Federation of Asian and Oceanian Biochemists and Molecular Biologists (FAOBMB)·Mahfuzul M HoqueI Aini
Apr 26, 2003·Avian Diseases·Olga Zorman-RojsEgbert Mundt
Nov 17, 2004·Avian Pathology : Journal of the W.V.P.A·T P van den BergH Müller
Jun 1, 2000·Avian Pathology : Journal of the W.V.P.A·T P Berg

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Citations

Apr 21, 2012·Avian Pathology : Journal of the W.V.P.A·Hermann MüllerM Rafiqul Islam
Jul 24, 2007·Journal of Virological Methods·Yu-Ching LeeYi-Yuan Yang
Nov 21, 2007·Nature Structural & Molecular Biology·Leremy A ColfK Christopher Garcia
Aug 14, 2018·Frontiers in Veterinary Science·Lorena Bustamante-CórdovaJesús Hernández

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