Chicken semen cryopreservation and use for the restoration of rare genetic resources

Poultry Science
Aurore ThélieE Blesbois

Abstract

For the past 50 yr, practices for ex situ preservation of endangered breeds have been extended. Semen and primordial germ cells, gonadic tissues have been frozen to create genetic stocks of chicken genetic diversity in cryobanks. Semen cryopreservation stays the preferred method since it is not invasive. Many protocols have been developed to cryopreserve chicken semen, but they give highly variable success rate. The aim of the present study was to standardize and prove the effectiveness of semen long-term storage for the restitution of lost families. We showed that semen straws stored for 18 yr in liquid nitrogen did not lose their fertilizing ability. We demonstrated the usefulness of cryopreserved semen stored in the French National Cryobank for the recovery of families of a subfertile experimental chicken line. In order to highlight the standardization of the cryopreserved method, different cryoprotectant protocols were also tested on a rare breed, freezing/thawing and insemination conditions were controlled. The best results were obtained using glycerol protocol, a sperm dilution of 1:4 (semen:extender). The insemination dose of 200 million sperm/female was as efficient as 400 million of sperm. Altogether, these results dem...Continue Reading

Citations

Mar 7, 2020·Animals : an Open Access Journal From MDPI·Michele Di IorioNicolaia Iaffaldano
Aug 6, 2020·Animals : an Open Access Journal From MDPI·Michele Di IorioNicolaia Iaffaldano
Sep 6, 2020·In Vitro Cellular & Developmental Biology. Animal·Juanjuan LiuHejun Du
Mar 5, 2021·Vavilovskiĭ Zhurnal Genetiki I Selekt︠s︡ii·Y L SilyukovaN V Dementieva
Jul 3, 2021·Animals : an Open Access Journal From MDPI·Lingwei SunDefu Zhang
Aug 28, 2021·Animals : an Open Access Journal From MDPI·Annelisse CastilloMargherita Marzoni Fecia di Cossato

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