Chikusetsusaponin IVa methyl ester induces cell cycle arrest by the inhibition of nuclear translocation of β-catenin in HCT116 cells

Biochemical and Biophysical Research Communications
Kyung-Mi LeeYeong Shik Kim

Abstract

We demonstrate that chikusetsusaponin IVa methyl ester (CME), a triterpenoid saponin from the root of Achyranthes japonica, has an anticancer activity. We investigate its molecular mechanism in depth in HCT116 cells. CME reduces the amount of β-catenin in nucleus and inhibits the binding of β-catenin to specific DNA sequences (TCF binding elements, TBE) in target gene promoters. Thus, CME appears to decrease the expression of cell cycle regulatory proteins such as Cyclin D1, as a representative target for β-catenin, as well as CDK2 and CDK4. As a result of the decrease of the cell cycle regulatory proteins, CME inhibits cell proliferation by arresting the cell cycle at the G0/G1 phase. Therefore, we suggest that CME as a novel Wnt/β-catenin inhibitor can be a putative agent for the treatment of colorectal cancers.

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Citations

Nov 9, 2016·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Xin ChenJin-Jian Lu
Jul 12, 2019·Biopharmaceutics & Drug Disposition·Wenzhou ZhangChongfeng Liu
Jan 17, 2020·Metabolites·Mohammed S HifnawyUsama Ramadan Abdelmohsen
Aug 5, 2017·Evidence-based Complementary and Alternative Medicine : ECAM·Hang ChenJin Fang
May 1, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Sojeong KimSang-Jip Nam

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