Children with atopic histories exhibit impaired lipopolysaccharide-induced Toll-like receptor-4 signalling in peripheral monocytes

Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology
D PréfontaineB D Mazer

Abstract

The hygiene hypothesis states that early exposure to bacterial products such as lipopolysaccharide (LPS) may be protective against the development of allergic diseases. Whether atopic disease affects the ability of immune cells to respond to LPS is unclear. Our laboratory has demonstrated previously that children express high levels of Toll-like receptor (TLR)-4 on CD4(+) cells in nasal mucosa. To determine if children with a history of allergic disease have impaired responses to LPS on circulating CD4(+) leucocytes. Peripheral blood mononuclear cells from children (aged 2-18) and adults with or without a history of atopic conditions were cultured with/without IL-4 or LPS for up to 24 h. Expression of surface TLR-4, CD14, CD4, CD3, as well as of intracellular phosphorylated (p42/p44) ERK and p38 mitogen-activated protein kinase (MAPK) were assessed by flow cytometry. A history of atopy in children was associated with impaired LPS-induced TLR-4-dependent phosphorylation of (p42/44) ERK and p38 MAPK by CD4(+) monocytes. Decreased LPS signalling was reproduced by pre-incubation of control cells with recombinant IL-4. LPS stimulation also decreased TLR-4 expression on monocytes from children without atopic histories but not from at...Continue Reading

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Citations

Jan 10, 2012·Current Opinion in Pediatrics·Anna B Fishbein, Ramsay L Fuleihan
Feb 19, 2011·American Journal of Respiratory Cell and Molecular Biology·Dane Parker, Alice Prince
Nov 24, 2011·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·C H KatelarisP Wark
Nov 23, 2013·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·L E P M van der VlugtH H Smits
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Sep 27, 2012·Current Opinion in Gastroenterology·Shannon L Russell, Brett B Finlay
Dec 30, 2014·Current Opinion in Allergy and Clinical Immunology·Koa HosokiSanjiv Sur
Jul 19, 2012·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·T HigakiK Nishizaki
Feb 25, 2017·Current Allergy and Asthma Reports·Regina K Rowe, Michelle A Gill
Aug 30, 2017·Frontiers in Immunology·Anastasia Georgountzou, Nikolaos G Papadopoulos
Jul 22, 2021·Pediatric Allergy and Immunology : Official Publication of the European Society of Pediatric Allergy and Immunology·Anastasia GeorgountzouNikolaos G Papadopoulos

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