Chill sensitivity and cryoprotectant permeability of dechorionated zebrafish embryos, Brachydanio rerio

Cryobiology
M HagedornW R Rall

Abstract

The zebrafish (Brachydanio rerio) was used as a model for basic studies of the chilling sensitivity, permeability and toxicity of cryoprotectants. In both intact and dechorionated embryos, early-stage embryos (1.25, 1.5, 1.75, and 2 h) were more susceptible (P < 0.05) to chilling injury at 0 degrees C than late-stage embryos (50, 75, and 100% epiboly and three-somite stage). Moreover, enzymatic removal of the chorion did not alter (P > 0.05) this pattern of sensitivity to chilling. Eight-hour zebrafish embryos tolerated short-term exposures to temperatures ranging from 4 to 23 degrees C for 3.5 h with no detrimental developmental effects. The permeability of dechorionated embryos to cryoprotectants was examined by measuring the kinetics of volumetric change at various developmental stages (16 cells to six somites or ca. 1.25 to 14 h postfertilization) at 28.5 degrees C. The dechorionated zebrafish embryo is composed of two complex cellular compartments (i.e., a large yolk and the developing blastoderm). From 40 to 100% epiboly, the volumes of yolk and blastoderm remained constant, ca. 82 and 18%, respectively. However, these volumes changed rapidly after epiboly. For example, at the six-somite stage, the yolk composed 61% of th...Continue Reading

References

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Citations

Jul 20, 2007·Zygote : the Biology of Gametes and Early Embryos·A Ninhaus-SilveiraR Veríssimo-Silveira
Nov 22, 2008·Zygote : the Biology of Gametes and Early Embryos·Alexandre Ninhaus-SilveiraRosicleire Veríssimo-Silveira
Oct 29, 2009·Zygote : the Biology of Gametes and Early Embryos·Violeta da Rocha PeriniNilo Bazzoli
Sep 19, 2009·Zebrafish·Vanesa RoblesM Paz Herráez
Jul 13, 2012·Zygote : the Biology of Gametes and Early Embryos·Patrícia Ribeiro NevesLeandro C Godoy
Jan 26, 2012·Cryobiology·K A AlmansooriN M Jomha
Jan 13, 2009·Cryobiology·S Martínez-PáramoM P Herráez
May 7, 2008·Cryobiology·S Martínez-PáramoM P Herráez
Sep 27, 2014·Zygote : the Biology of Gametes and Early Embryos·Arthur Vinícius Lourenço FerreiraCelia Maria de Souza Sampaio
Nov 19, 2013·Reproduction in Domestic Animals = Zuchthygiene·F ShalueiM H Nasr-Esfahani
Jun 7, 2005·Theriogenology·Delgado M ValdezMagosaburo Kasai
Dec 13, 2005·Cryobiology·M HagedornF W Kleinhans
Mar 13, 2002·Cryobiology·M KöseoğluK C Sadler
Apr 15, 2014·Cryobiology·M Mohd Sharifuddin, M N Siti Azizah
Feb 7, 2017·PloS One·Bernadett FaragóJudit Cserepes
Oct 22, 1998·Biology of Reproduction·M HagedornU Pilatus
Feb 5, 2009·Reproduction in Domestic Animals = Zuchthygiene·K A El-Battawy, O Linhart
Feb 7, 2018·Cryobiology·Fatemeh JalaliJens O M Karlsson

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