Chilling susceptibility in mungbean varieties is associated with their differentially expressed genes

Botanical Studies
Li-Ru ChenTsai-Yun Lin

Abstract

Mungbean (Vigna radiata L. Wilczek) is an economically important legume of high nutritional value, however, its cultivation is limited by susceptibility to chilling. Varieties NM94 and VC1973A, with differential susceptibility to stress, serve as good materials for uncovering how they differ in chilling tolerance. This study aimed to identify the ultrastructural, physiological and molecular changes to provide new insights on the differential susceptibility to chilling between varieties VC1973A and NM94. Chilling stress caused a greater reduction in relative growth rate, a more significant decrease in maximum photochemical efficiency of PSII and DPPH scavenging activity and more-pronounced ultrastructural changes in VC1973A than in NM94 seedlings. Comparative analyses of transcriptional profiles in NM94 and VC1973A revealed that the higher expression of chilling regulated genes (CORs) in NM94. The transcript levels of lipid transfer protein (LTP), dehydrin (DHN) and plant defensin (PDF) in NM94 seedlings after 72 h at 4 °C was higher than that in its parental lines VC1973A, 6601 and VC2768A. Our results suggested that LTP, DHN and PDF may mediate chilling tolerance in NM94 seedlings.

References

Sep 30, 2000·European Journal of Biochemistry·E S Arnér, A Holmgren
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jan 17, 2003·Plant Physiology·Myong-Chul KoagTimothy J Close
Mar 17, 2004·DNA Sequence : the Journal of DNA Sequencing and Mapping·Kun-Hsiang Liu, Tsai-Yun Lin
Aug 21, 2009·Annual Review of Genetics·Yves MeyerJean-Philippe Reichheld
Apr 14, 2010·Methods in Molecular Biology·Ananda MustafizSudhir K Sopory
Sep 9, 2010·Nature Reviews. Molecular Cell Biology·Sima Lev
Apr 19, 2011·Functional & Integrative Genomics·Annie Archambault, Martina V Strömvik
Mar 23, 2012·Journal of Plant Physiology·Tahsina Sharmin HoqueYoshiyuki Murata
Mar 24, 2012·Plant & Cell Physiology·Chia-Hui LinTsai-Yun Lin
Mar 8, 2013·International Journal of Molecular Sciences·Kenji Miura, Tsuyoshi Furumoto
Aug 26, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Kim VriensKarin Thevissen
Nov 12, 2014·Nature Communications·Yang Jae KangSuk-Ha Lee

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Citations

Oct 26, 2020·Journal of Pharmaceutical and Biomedical Analysis·Admassu Assen AdemReinhard H H Neubert

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Methods Mentioned

BETA
transmission electron microscopy
PCR

Software Mentioned

Primer Express
Nikon NIS
- element
CoStat

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