Chimeras of channelrhodopsin-1 and -2 from Chlamydomonas reinhardtii exhibit distinctive light-induced structural changes from channelrhodopsin-2.

The Journal of Biological Chemistry
Asumi InagumaYuji Furutani

Abstract

Channelrhodopsin-2 (ChR2) from the green alga Chlamydomonas reinhardtii functions as a light-gated cation channel that has been developed as an optogenetic tool to stimulate specific nerve cells in animals and control their behavior by illumination. The molecular mechanism of ChR2 has been extensively studied by a variety of spectroscopic methods, including light-induced difference Fourier transform infrared (FTIR) spectroscopy, which is sensitive to structural changes in the protein upon light activation. An atomic structure of channelrhodopsin was recently determined by x-ray crystallography using a chimera of channelrhodopsin-1 (ChR1) and ChR2. Electrophysiological studies have shown that ChR1/ChR2 chimeras are less desensitized upon continuous illumination than native ChR2, implying that there are some structural differences between ChR2 and chimeras. In this study, we applied light-induced difference FTIR spectroscopy to ChR2 and ChR1/ChR2 chimeras to determine the molecular basis underlying these functional differences. Upon continuous illumination, ChR1/ChR2 chimeras exhibited structural changes distinct from those in ChR2. In particular, the protonation state of a glutamate residue, Glu-129 (Glu-90 in ChR2 numbering), i...Continue Reading

References

Jun 28, 1977·Biochemistry·B AtonT G Ebrey
Jun 13, 2002·Proceedings of the National Academy of Sciences of the United States of America·Oleg A SineshchekovJohn L Spudich
Jun 29, 2002·Science·Georg NagelPeter Hegemann
Dec 24, 2002·Biophysical Journal·Yuji FurutaniHideki Kandori
Feb 5, 2003·Biochemical and Biophysical Research Communications·Takeshi SuzukiTetsuo Takahashi
Nov 15, 2003·Proceedings of the National Academy of Sciences of the United States of America·Georg NagelErnst Bamberg
Aug 24, 2005·Nature Neuroscience·Edward S BoydenKarl Deisseroth
Sep 23, 2006·Nature Methods·Feng ZhangKarl Deisseroth
Nov 27, 2007·Journal of Molecular Biology·Christian BamannErnst Bamberg
Dec 17, 2008·Nature Neuroscience·André BerndtKarl Deisseroth
Dec 24, 2008·The Journal of Biological Chemistry·Hongxia WangHiromu Yawo
May 9, 2009·Journal of the American Chemical Society·Ionela RaduJ Heberle
Oct 29, 2009·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Ionela RaduJ Heberle
Feb 4, 2010·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Melanie NackJ Heberle
Apr 21, 2011·Proceedings of the National Academy of Sciences of the United States of America·André BerndtThomas G Oertner
Jan 6, 2012·The Journal of Biological Chemistry·Kirstin EisenhauerKlaus Gerwert
Jan 24, 2012·Nature·Hideaki E KatoOsamu Nureki
Mar 20, 2013·Proceedings of the National Academy of Sciences of the United States of America·Víctor A Lórenz-FonfríaJ Heberle
Sep 24, 2013·Biochimica Et Biophysica Acta·Klaus GerwertSteffen Wolf
Nov 12, 2013·Biochimica Et Biophysica Acta·Víctor A Lórenz-Fonfría, J Heberle
Feb 12, 2014·Journal of the American Chemical Society·Shota ItoHideki Kandori
Mar 29, 2014·Science·Jonas WietekPeter Hegemann
Dec 30, 2014·Angewandte Chemie·Jens KuhneFranz Bartl
Oct 18, 2012·The Journal of Physical Chemistry Letters·Yuji FurutaniHideki Kandori

Citations

Nov 18, 2015·Journal of Chemical Theory and Computation·Dalibor HršakChristof Hättig
Sep 10, 2015·Physical Chemistry Chemical Physics : PCCP·I Dokukina, O Weingart
Mar 17, 2018·The Journal of Biological Chemistry·Hisao TsukamotoYuji Furutani
Nov 25, 2017·Science·Oleksandr VolkovValentin Gordeliy
Mar 21, 2018·Proceedings of the National Academy of Sciences of the United States of America·Albert Ardevol, Gerhard Hummer
Apr 13, 2018·Frontiers in Molecular Neuroscience·Wu ZhengJiang-Fan Chen
Mar 24, 2020·Chemical Reviews·Víctor A Lórenz-Fonfría
Sep 6, 2020·Progress in Neuro-psychopharmacology & Biological Psychiatry·Marc Fakhoury

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Related Papers

Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology
Melanie NackJ Heberle
The Journal of Biological Chemistry
Hiroshi WatanabeMarcus Elstner
© 2021 Meta ULC. All rights reserved