Chimeric Adeno-Associated Virus-Mediated Cardiovascular Reprogramming for Ischemic Heart Disease

ACS Omega
So Young YooSeung-Wuk Lee

Abstract

Here, we demonstrated chimeric adeno-associated virus (chimeric AAV), AAV-DJ-mediated cardiovascular reprogramming strategy to generate new cardiomyocytes and limit collagen deposition in cardiac fibroblasts by inducing synergism of chimeric AAV-expressing Gata4, Mef2c, Tbx5 (AAV-GMT)-mediated heart reprogramming and chimeric AAV-expressing thymosin β4 (AAV-Tβ4)-mediated heart regeneration. AAV-GMT promoted a gradual increase in expression of cardiac-specific genes, including Actc1, Gja1, Myh6, Ryr2, and cTnT, with a gradual decrease in expression of a fibrosis-specific gene, procollagen type I and here AAV-Tβ4 help to induce GMT expression, providing a chimeric AAV-mediated therapeutic cell reprogramming strategy for ischemic heart diseases.

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Citations

Nov 5, 2019·Journal of Tissue Engineering and Regenerative Medicine·Paula García-OlloquiBeatriz Pelacho
Sep 22, 2020·EMBO Molecular Medicine·Mark SweeneyStuart A Cook
Nov 14, 2019·Cancers·So Young YooDae-Hwan Kang
Sep 26, 2020·Biotechnology Journal·Jihad El Andari, Dirk Grimm
Oct 22, 2020·International Journal of Molecular Sciences·Lin JiangYigang Wang
Jul 3, 2021·Seminars in Cell & Developmental Biology·Taketaro Sadahiro, Masaki Ieda
Jun 22, 2021·Frontiers in Bioengineering and Biotechnology·Mira ChingaleKe Huang

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Datasets Mentioned

BETA
GMT

Methods Mentioned

BETA
PCR

Software Mentioned

AAV
Image J
Image Pro
Helper

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