Chimeric Fc receptors identify immunoglobulin-binding regions in human Fc gamma RII and Fc epsilon RI

European Journal of Immunology
M D HulettP M Hogarth

Abstract

Fc gamma RII and Fc epsilon RI are functionally distinct cell surface receptors for immunoglobulin (Ig); Fc gamma RII binds IgG with low affinity, whereas Fc epsilon RI binds IgE with high affinity, yet they are homologous in structure and sequence having extracellular regions containing two Ig-like domains with 38% amino acid identity. Chimeric receptors derived from human Fc gamma RII and Fc epsilon RI were produced by exchanging homologous regions of the two receptors to define binding region(s) for IgG in Fc gamma RII and IgE in Fc epsilon RI. Firstly, a chimeric form of the Fc epsilon RI alpha chain was produced by replacing the transmembrane region and cytoplasmic tail with that of Fc gamma RII. This mutant alpha chain could be expressed on the cell surface independently of associated beta and gamma subunits, and retained high-affinity IgE binding, indicating that the extracellular region of the Fc epsilon RI alpha chain is sufficient for high-affinity IgE binding. Secondly, to identify the role of the individual domains in Fc binding of both Fc gamma RII and Fc epsilon RI, chimeric receptors were generated by exchanging the first extracellular domains between Fc gamma RII and the alpha chain mutant and used to demonstrat...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
May 1, 1992·The Journal of Experimental Medicine·T BieberC de la Salle
May 1, 1992·The Journal of Experimental Medicine·B WangG Stingl
May 1, 1991·Journal of Leukocyte Biology·J G van de Winkel, C L Anderson
Jan 1, 1991·Annual Review of Immunology·J V Ravetch, J P Kinet
Jul 1, 1990·The Journal of Experimental Medicine·P A WarmerdamP J Capel
Jan 1, 1985·Immunogenetics·M L HibbsI F McKenzie
May 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·B Seed, A Aruffo
Mar 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·A ShimizuP Leder
Apr 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M L HibbsP M Hogarth
Jan 1, 1988·Annual Review of Immunology·J C UnkelessV H Freedman
Jan 1, 1986·Annual Review of Immunology·H MetzgerR Quarto
Jan 1, 1988·Annual Review of Immunology·A F Williams, A N Barclay
Nov 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·K L HolmesH C Morse
Jan 1, 1983·Annual Review of Immunology·D R Davies, H Metzger
Jan 1, 1980·Immunogenetics·F W Shen, E A Boyse

❮ Previous
Next ❯

Citations

Jul 25, 2000·Journal of Clinical Laboratory Analysis·B K Flesch, J Neppert
Apr 17, 2002·Molecular Immunology·Beth A Wurzburg, Theodore S Jardetzky
Nov 5, 2002·Current Opinion in Immunology·Howard R Katz
Oct 4, 2011·The Journal of Biological Chemistry·Jinghua LuPeter D Sun
Jun 8, 1999·Annual Review of Immunology·J P Kinet
Jun 8, 1999·Annual Review of Immunology·S C GarmanT S Jardetzky
Jun 11, 2002·Biochemical and Biophysical Research Communications·Akio IwasakiTakao Nagoya
Jan 6, 1999·Cell·S C GarmanT S Jardetzky
Jul 15, 2004·International Immunopharmacology·Matthias BudtHartmut Hengel
May 7, 1999·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·I Sayers, B A Helm
Dec 2, 1993·Nature·B J Sutton, H J Gould
Sep 8, 1995·The Journal of Biological Chemistry·M D HulettP M Hogarth
May 1, 1999·The Journal of Biological Chemistry·M D HulettP M Hogarth
Aug 23, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Paul A RamslandP Mark Hogarth
Jan 1, 1997·International Reviews of Immunology·M Daëron
Dec 18, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Halina M TristP Mark Hogarth
Jan 1, 1997·International Reviews of Immunology·A Tamm, R E Schmidt
Jul 6, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·M P RudolfF Kricek
Feb 2, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Graham A MackayHannah J Gould
Aug 4, 2007·Molecular Immunology·Angela C CendronP Mark Hogarth

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.