PMID: 11316328Apr 24, 2001Paper

Chimeric Galphaq subunits can distinguish the long form of the Xenopus Mel1c melatonin receptor from the mammalian mt1 and MT2 melatonin receptors

Journal of Pineal Research
F P LaiY H Wong

Abstract

The family of melatonin receptors is composed of the mt1, MT2, and Mel1c subtypes. The Mel1c is further divided into one long and two short isoforms. A recent study has shown that, unlike mt1 and MT2, the long form of Mel1c is incapable of activating the pertussis toxin-insensitive G16. Here we used three well-characterized Galphaq chimeras to explore the coupling specificity of the melatonin receptors. The qi5, qo5, and qz5 chimeras can link numerous Gi-coupled receptors to the stimulation of phosphoinositide-specific phospholipase C. Both mt1 and MT2 receptors interacted productively with the Galphaq chimeras, while the long form of Mel1c was totally ineffective. Among the Galphaq chimeras, qo5 was less efficiently coupled to the melatonin receptors. Such differential coupling is best explained by structural differences between the melatonin receptors as well as among the Galphaq chimeras. Since the long form of Mel1c receptor possesses an exceptionally large C-terminal tail, we tested the ability of four melatonin receptor C-terminal tail chimeras (Chi 1-4) to interact with the Galphaq chimeras. The presence of the large C-terminal tail of Mel1c in Chi 1 and Chi 3 markedly hindered their coupling to the Galphaq chimeras. On ...Continue Reading

Citations

Apr 17, 2003·Neuroscience Letters·Prapapun ChucharoenPiyarat Govitrapong
Aug 3, 2004·Assay and Drug Development Technologies·David C New, Yung H Wong
Aug 24, 2011·World Journal of Diabetes·Javier EspinoAna B Rodríguez
Sep 10, 2005·Journal of Pineal Research·Andreas G BachElmar Peschke
Jan 31, 2006·Journal of Pineal Research·Elmar PeschkeEckhard Mühlbauer
Dec 15, 2007·Journal of Pineal Research·Elmar Peschke
Apr 24, 2015·Journal of Pineal Research·Elmar PeschkeEckhard Mühlbauer
Mar 29, 2013·International Journal of Molecular Sciences·Elmar PeschkeEckhard Mühlbauer

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