Chimeric GII.3/GII.6 norovirus capsid (VP1) proteins: characterization by electron microscopy, trypsin sensitivity and binding to histo-blood group antigens

Archives of Virology
Shuhuan MaShuo Shen

Abstract

GII.3 and GII.6 noroviruses (NoVs) are similar in several aspects, including the presence of a short sequence insertion in the P2 domain of the major capsid protein (VP1) and trypsin susceptibility of VP1-containing virus-like particles (VLPs). In this study, we generated two constructs with the S or P domains of VP1 from GII.3 and GII.6 NoV strains exchanged (GII.3S/GII.6P and GII.6S/GII.3P), and the resultant chimeric capsid proteins were expressed from recombinant baculoviruses. The assembly of VLPs was confirmed by electron microscopy, and the susceptibility of assembled VLPs to trypsin digestion was analyzed by SDS-PAGE. Salivary histo-blood group antigen (HBGA) binding and binding blockade assays were performed to determine the binding characteristics of chimeric VP1-containing VLPs with and without trypsin digestion. Our results indicated that both expressed GII.3S/GII.6P and GII.6S/GII.3P chimeric proteins successfully assembled into VLPs. Trypsin digestion of VLPs assembled from both chimeric proteins led to the generation of two fragments with molecular sizes similar to those of wild-type VP1-containing VLPs. An in vitro salivary HBGA binding assay demonstrated that VLPs assembled from both chimeric proteins exhibited...Continue Reading

References

Aug 1, 1994·Journal of Virology·B V PrasadM K Estes
Oct 9, 1999·Science·B V PrasadM K Estes
Sep 28, 2005·The Journal of General Virology·Vance P LochridgeMichele E Hardy
Feb 15, 2008·PLoS Medicine·Lisa C LindesmithRalph S Baric
Aug 15, 2008·Journal of Virology·Haruko ShiratoNaokazu Takeda
Oct 8, 2008·Immunological Reviews·Eric F DonaldsonRalph S Baric
Jul 1, 2011·Journal of Virology·Denali BoonKarin Bok
Jan 24, 2012·Journal of Virological Methods·Tiia KohoVesa P Hytönen
Aug 3, 2013·The Journal of Infectious Diseases·Kari DebbinkRalph S Baric
Mar 29, 2014·PloS One·Julia Monassa FiorettiMarize Pereira Miagostovich
Jul 2, 2014·The Lancet Infectious Diseases·Sharia M AhmedBenjamin A Lopman
Jul 6, 2014·Journal of Clinical Microbiology·Jan Vinjé
Feb 11, 2015·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Liang XueWeipeng Guo
Apr 22, 2015·Virus Research·Yuqi HuoShuo Shen
Oct 30, 2015·Nature Protocols·Melissa K JonesStephanie M Karst
Aug 2, 2016·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Jones Anderson Monteiro SiqueiraYvone Benchimol Gabbay
Aug 27, 2016·Science·Khalil EttayebiMary K Estes
Oct 7, 2017·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Filemón BucardoJohan Nordgren
Jan 10, 2018·Frontiers in Microbiology·Erin A AlmandLee-Ann Jaykus

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Datasets Mentioned

BETA
KY767665
AB818400

Methods Mentioned

BETA
enzyme-linked immunosorbent assay
electrophoresis
electron microscopy
PCR
ELISA

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