Chimeric rhinoviruses as tools for vaccine development and characterization of protein epitopes

Intervirology
G F ArnoldE Arnold

Abstract

Chimeric human rhinoviruses (HRVs) have the potential to serve as vaccines against a wide variety of diseases. Such vaccines can be developed optimally by generating libraries of chimeric HRVs displaying immunogens from dangerous pathogens or tumor cells in many different conformations. Extremely large numbers of conformationally defined presentations of foreign epitopes can be produced efficiently by flanking transplanted epitopes with linkers, or adapters, of small segments of randomized amino acids. In addition, the individual residues of the immunogenic sequences can be encoded in proportion to their prevalence in databases, generating composite immunogens that function as mimotopes. The diversity of sequences and conformations improves the likelihood of generating immunologically valuable vaccine candidates. Chimeric viruses thus generated can be propagated and purified to select for viruses whose growth and physical stability are like those of wild-type HRV. Viruses containing a foreign epitope in antigenically relevant conformations can then be captured by immunoselection with neutralizing antibodies directed against the foreign pathogen. Using this approach, we have been able to generate HRV chimeras that present V3 loo...Continue Reading

Citations

Apr 25, 2000·Journal of Virology·K HöflingJ Smith Leser
Jun 27, 2001·FEMS Microbiology Letters·M J Schnell
Aug 7, 2002·Journal of Virology·Andrew T DufresneMatthias Gromeier
Feb 19, 1999·Journal of Clinical Pathology·J S OxfordR Lambkin
Mar 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·P A KratzM Nassal
Feb 26, 2016·Viruses·Jani Ylä-PeltoPetri Susi
Sep 25, 2002·Clinics in Laboratory Medicine·James P McGettiganMatthias J Schnell

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