Chimeric subgroup A respiratory syncytial virus with the glycoproteins substituted by those of subgroup B and RSV without the M2-2 gene are attenuated in African green monkeys

Virology
X ChengH Jin

Abstract

Using the existing reverse genetics system developed for the subgroup A respiratory syncytial virus (RSV), a chimeric virus (designated rA-G(B)F(B)) that expresses subgroup B-specific antigens was constructed by replacing the G and F genes of the A2 strain with those of the 9320 strain of subgroup B RSV. rA-G(B)F(B) grew well in tissue culture, but it was attenuated in the respiratory tracts of cotton rats and African green monkeys. To further attenuate this chimeric RSV, the M2-2 open reading frame was removed from rA-G(B)F(B). rA-G(B)F(B)DeltaM2-2 was highly attenuated in replication in the respiratory tracts of the infected monkeys, but it provided complete protection against wild-type subgroup B RSV challenge following two doses of infection. In this study, rA2DeltaM2-2 (a recombinant A2 RSV that lacks the M2-2 gene) was also evaluated in African green monkeys. The replication of rA2DeltaM2-2 was highly restricted in both the upper and lower respiratory tracts of the infected monkeys and it induced titers of serum anti-RSV neutralizing antibody that were slightly lower than those induced by wild-type rA2. When rA2DeltaM2-2-infected monkeys were challenged with wild-type A2 virus, the replication of the challenge virus was r...Continue Reading

References

Nov 1, 1979·The Journal of Infectious Diseases·J C Hierholzer, M S Hirsch
Jun 1, 1976·The Journal of Pediatrics·P F WrightD T Karzon
Mar 1, 1990·Archives of Disease in Childhood·H A van Steensel-MollJ A Hazelzet
Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·P R JohnsonP L Collins
Apr 1, 1974·Proceedings of the Society for Experimental Biology and Medicine·D S HodesR M Chanock
Apr 1, 1969·Journal of Virology·M A GharpureR M Chanock
Mar 14, 1998·Journal of Virology·P SpielhoferH Y Naim
Sep 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·A Bermingham, P L Collins
Jan 11, 2000·Clinical Microbiology Reviews·W M Sullender

❮ Previous
Next ❯

Citations

Mar 20, 2010·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Kerry M EmpeyJay K Kolls
Feb 7, 2013·Viruses·Rajeev RudrarajuJulia L Hurwitz
Aug 28, 2007·Comparative Immunology, Microbiology and Infectious Diseases·Gilles MeyerFrançois Schelcher
Dec 3, 2003·Journal of Evolutionary Biology·C L BurchK A Hanley
Oct 22, 2002·The Journal of General Virology·Gabriele NeumannYoshihiro Kawaoka
Oct 5, 2013·The Journal of General Virology·Heather A LawlorRoderick S Tang
Sep 24, 2013·Expert Review of Vaccines·Vanessa KurzweilMark T Esser
Sep 19, 2002·The Pediatric Infectious Disease Journal·Martin C J Kneyber, Jan L L Kimpen

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.