Chimeric symbionts expressing a Wolbachia protein stimulate mosquito immunity and inhibit filarial parasite development.

Communications Biology
Sara EpisClaudio Bandi

Abstract

Wolbachia can reduce the capability of mosquitoes to transmit infectious diseases to humans and is currently exploited in campaigns for the control of arboviruses, like dengue and Zika. Under the assumption that Wolbachia-mediated activation of insect immunity plays a role in the reduction of mosquito vectorial capacity, we focused our attention on the Wolbachia surface protein (WSP), a potential inductor of innate immunity. We hypothesized that the heterologous expression of this protein in gut- and tissue-associated symbionts may reduce parasite transmission. We thus engineered the mosquito bacterial symbiont Asaia to express WSP (AsaiaWSP). AsaiaWSP induced activation of the host immune response in Aedes aegypti and Anopheles stephensi mosquitoes, and inhibited the development of the heartworm parasite Dirofilaria immitis in Ae. aegypti. These results consolidate previous evidence on the immune-stimulating property of WSP and make AsaiaWSP worth of further investigations as a potential tool for the control of mosquito-borne diseases.

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Citations

Sep 10, 2020·Frontiers in Microbiology·Matteo CallegariElena Crotti
Oct 24, 2020·Tropical Medicine and Infectious Disease·Elerson Matos RochaJayme A Souza-Neto
Jan 15, 2021·Scientific Reports·Leonardo M Díaz-NietoCorina M Berón
Apr 16, 2021·Applied Microbiology and Biotechnology·Philipp Moritz FrickeTino Polen
May 9, 2021·Parasites & Vectors·Ranju Ravindran Santhakumari ManojDomenico Otranto
Jun 10, 2021·Trends in Microbiology·Katherine M ElstonJeffrey E Barrick
Nov 8, 2020·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Ilaria Varotto-BoccazziClaudio Bandi
Aug 29, 2021·Developmental and Comparative Immunology·Cybèle Prigot-MauriceChristine Braquart-Varnier

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Methods Mentioned

BETA
PCR
transgenic
phosphotranferase

Software Mentioned

RStudio
GraphPad Prism
ImageJ

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