PMID: 9551366Apr 29, 1998Paper

Chimpanzee dendritic cells derived in vitro from blood monocytes and pulsed with antigen elicit specific immune responses in vivo

Journal of Immunotherapy
Simon M Barratt-BoyesO J Finn

Abstract

Dendritic cells differentiated in vitro from blood and other sources using cytokines hold particular promise as immunotherapeutic agents in cancer. However, there are currently no data to show that human in vitro-derived dendritic cells are immunogenic in vivo. We have developed a primate model of immunotherapy using dendritic cells differentiated in vitro from blood monocytes by culturing with human granulocyte/ macrophage colony-stimulating factor and interleukin-4. We measured the immune response to antigen elicited by in vitro-derived and antigen-treated dendritic cells following a single intravenous inoculation an boost in chimpanzees. The antigens tested were ovalbumin, a complex foreign protein, and a peptide derived from the MUC-1 mucin tumor antigen, a relatively uncomplex self antigen. Four chimpanzees were immunized either with antigen-pulsed dendritic cells (two animals) or mock-treated dendritic cells (one animal) given intravenously or both antigens given in adjuvant subcutaneously (one animal). Each animal received a boost of both antigens in adjuvant 10 days later. All animals responded with an IgG-mediated humoral response to ovalbumin measured in the serum at day 24. This was associated with a proliferative ce...Continue Reading

Citations

Oct 10, 2002·Biochemical and Biophysical Research Communications·Kejian ZhuUlrich Mrowietz
Mar 24, 1999·Molecular Medicine Today·C A Colaco
Sep 22, 2001·Journal of Immunotherapy·K MaruyamaK Yamaguchi
Feb 17, 2001·The Journal of Investigative Dermatology·K Zhu, U Mrowietz
Feb 15, 2000·Cytokine·M A Antonysamy, A W Thomson
Oct 8, 2009·Alternatives to Laboratory Animals : ATLA·Jarrod Bailey
Oct 2, 2008·Alternatives to Laboratory Animals : ATLA·Jarrod Bailey

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