PMID: 9175253May 1, 1997Paper

Chinese hamster ovary cells are non-permissive towards infection with coxsackievirus B3 despite functional virus-receptor interactions

Virus Research
B KramerH C Selinka

Abstract

Viral infection is a complex process which includes binding and interaction of the virus with specific cell surface receptors, uptake and uncoating of the virus, and finally replication. Chinese hamster ovary (CHO) cells are non-permissive towards infection with coxsackieviruses of group B (CVB), although they do express a putative CVB-specific receptor protein. In order to localize the block of infection in CHO cells, these cells were tested for binding of radiolabelled CVB3, receptor-mediated transformation of virions into A-particles, and replication of the viral genome. Binding of CVB3 to CHO cells was found to be comparable to the binding of this virus to permissive cell lines. Detergent-solubilized membrane proteins of CHO cells were tested in virus overlay protein-binding assays (VOPBAs) and shown to express a 100 kDa CVB-binding membrane protein similar to the CVB receptor protein which we recently described for permissive HeLa cells. Incubation of CVB3 with intact CHO cells resulted in transformation of cell-bound virions into non-infectious A-particles (deprived of capsid protein VP4), demonstrating the functional activity of the CVB receptor protein on CHO hamster cells. Transfection of recombinant CVB3 cDNA or viral...Continue Reading

References

Feb 26, 1976·Nature·K Lonberg-HolmL Philipson
Jul 1, 1992·Virus Research·D W LinebergerR J Colonno
May 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·H C SelinkaE Wimmer
Jan 1, 1990·Archives of Virology·D SawitzkyK O Habermehl
Jul 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·R Kandolf, P H Hofschneider
Mar 10, 1989·Cell·J M GreveA McClelland
Oct 1, 1988·The Journal of General Virology·H MischakD Blaas
Feb 1, 1985·Journal of Molecular and Cellular Cardiology·R KandolfP H Hofschneider
Jan 1, 1973·Annual Review of Medicine·W H Abelmann
Jul 1, 1971·Proceedings of the Society for Experimental Biology and Medicine·R L CrowellL Philipson
Jun 21, 1994·Proceedings of the National Academy of Sciences of the United States of America·J M BergelsonR W Finberg
Mar 15, 1994·Proceedings of the National Academy of Sciences of the United States of America·M ManchesterM B Oldstone
Aug 1, 1993·Clinical Immunology and Immunopathology·R KandolfA Heim

❮ Previous
Next ❯

Citations

Jul 1, 1998·Clinical and Diagnostic Virology·H C SelinkaR Kandolf
Nov 28, 2007·Annual Review of Pathology·Mitra Esfandiarei, Bruce M McManus
Dec 3, 2014·International Journal of Cardiology·Chandirasegaran MassilamanyJay Reddy
Jan 5, 2005·Biochemical and Biophysical Research Communications·Shue-Ling RaungChun-Jung Chen

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.