Chinese Propolis Exerts Anti-Proliferation Effects in Human Melanoma Cells by Targeting NLRP1 Inflammatory Pathway, Inducing Apoptosis, Cell Cycle Arrest, and Autophagy

Nutrients
Yufei ZhengFuliang Hu

Abstract

Melanoma is a malignant tumor that begins in the melanocyte and has the highest mortality rate among all cutaneous tumors. Chinese propolis (CP) has been shown to have a potent antitumor effect against various cancers. In this study, we uncovered the combined effects of antiproliferation and anti-inflammation of CP on suppressing the progression of human melanoma cell line A375. We evaluated the alterations of protein expression after CP treatment by Western blot. After CP treatment, A375 cells underwent intrinsic apoptosis and cell cycle arrest. Furthermore, we found that CP suppressed inflammation in A375 cells. NLRP1 (NLR family pyrin domain containing 1), confirmed as a proinflammatory protein in melanoma progression, was downregulated significantly by CP, as were the NLRP1-related caspase activation and recruitment domains (CARD) proteins, including caspase-1 and caspase-4. Additionally, decreasing mRNA levels of IL-1α, IL-1β, and IL-18 further proved the negative regulation of CP on the melanoma inflammatory environment. We also discovered that CP induced autophagy in A375 cells. Interestingly, inhibiting autophagy in CP-treated cells diminished its antitumor effect, suggesting that the autophagy was attributed to CP-indu...Continue Reading

References

Jan 19, 2000·Proceedings of the National Academy of Sciences of the United States of America·F Vidal-VanaclochaC A Dinarello
Oct 16, 2001·Immunology Letters·H ParkD H Cho
May 25, 2002·Journal of Natural Products·Tepy UsiaShigetoshi Kadota
Dec 31, 2002·Toxicology·J A Pietenpol, Z A Stewart
Feb 25, 2003·Proceedings of the National Academy of Sciences of the United States of America·Elena VoronovRon N Apte
Mar 12, 2003·Trends in Pharmacological Sciences·Zoe A StewartJennifer A Pietenpol
May 12, 2004·Current Drug Targets·Jason S Damiano, John C Reed
Jul 13, 2006·Cancer Immunology, Immunotherapy : CII·Penka N NikolovaElissaveta J Naumova
Jul 29, 2006·Autophagy·Jayanta DebnathGuido Kroemer
Sep 16, 2006·Cell Death and Differentiation·F Martinon, J Tschopp
Nov 18, 2006·Nature Immunology·Jörg H FritzStephen E Girardin
Jun 21, 2007·Journal of Ethnopharmacology·J M Sforcin
Mar 12, 2008·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Qiang ZhangZhu-Jun Wang
Mar 13, 2009·Journal of Natural Products·Na ShaDe-An Guo
Apr 17, 2009·Pigment Cell & Melanoma Research·Vladislava O Melnikova, Menashe Bar-Eli
Jul 21, 2009·Journal of Cutaneous Pathology·Rossitza LazovaJohn Pawelek
Mar 5, 2010·European Journal of Immunology·Katherine A Fitzgerald
Jun 2, 2010·Current Genomics·Kyle K Biggar, Kenneth B Storey
Oct 26, 2010·Journal of Ethnopharmacology·José Maurício Sforcin, Vassya Bankova
Jan 7, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Nao JounaiFumihiko Takeshita
Jan 14, 2011·The Journal of Biological Chemistry·James HarrisEd C Lavelle
Feb 12, 2011·Cell Death and Differentiation·R KangD Tang
Jul 1, 2008·Environmental Toxicology and Pharmacology·Muneo MotomuraCheorl-Ho Kim
Sep 29, 2011·Molecular Cancer Research : MCR·Yong QinElizabeth A Grimm
Oct 5, 2011·Proceedings of the National Academy of Sciences of the United States of America·Christiane MeyerViktor Umansky
Nov 5, 2011·Cell Death and Differentiation·D DentonS Kumar
Nov 15, 2011·Cell·Noboru Mizushima, Masaaki Komatsu
Nov 17, 2011·Nucleic Acids Research·Xiaowei WangBrian Seed
Mar 20, 2012·Cancer Management and Research·Anton G Kutikhin, Arseniy E Yuzhalin
May 29, 2012·Photochemistry and Photobiology·Tahseen H Nasti, Laura Timares
Jul 5, 2012·The Journal of Biological Chemistry·Maria Lucia MarinoAngelo De Milito
Aug 31, 2012·The Journal of Investigative Dermatology·Weimin LiuMayumi Fujita
Nov 8, 2012·PloS One·Amaia Marcilla-EtxenikeXavier Busquets
Sep 24, 2013·Lancet·Alexander M M EggermontCaroline Robert
May 6, 2014·Frontiers in Immunology·Irving Coy Allen

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Citations

Feb 27, 2020·Journal of Cosmetic Dermatology·Julie K NguyenJared Jagdeo
Jul 11, 2020·Journal of Cancer Prevention·Hui-Fang ChiuChin-Kun Wang
Apr 11, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xiasen JiangFuliang Hu
Aug 11, 2020·Evidence-based Complementary and Alternative Medicine : ECAM·Xia-Sen JiangFu-Liang Hu
Mar 9, 2021·Biochemistry and Biophysics Reports·Azza Hanif HarisnaEtik Mardliyati
Jun 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Lingchen TaoFuliang Hu
Aug 17, 2020·Journal of Agricultural and Food Chemistry·Zheng YufeiHu Fuliang

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Methods Mentioned

BETA
lipidation
flow cytometry
protein assay

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