Chip-based visual detection of microRNA using DNA-functionalized gold nanoparticles

Science China. Life Sciences
Ping WangJianlong Zhao

Abstract

In the present study, we developed a highly sensitive and convenient biosensor consisting of gold nanoparticle (AuNP) probes and a gene chip to detect microRNAs (miRNAs). Specific oligonucleotides were attached to the glass surface as capture probes for the target miRNAs, which were then detected via hybridization to the AuNP probes. The signal was amplified via the reduction of HAuCl4 by H2O2. The use of a single AuNP probe detected 10 pmol L(-1) of target miRNA. The recovery rate for miR-126 from fetal bovine serum was 81.5%-109.1%. The biosensor detection of miR-126 in total RNA extracted from lung cancer tissues was consistent with the quantitative PCR (qPCR) results. The use of two AuNP probes further improved the detection sensitivity such that even 1 fmol L(-1) of target miR-125a-5p was detectable. This assay takes less than 1 h to complete and the results can be observed by the naked eye. The platform simultaneously detected lung cancer related miR-126 and miR-125a-5p. Therefore, this low cost, rapid, and convenient technology could be used for ultrasensitive and robust visual miRNA detection.

References

Oct 1, 1996·Proceedings of the National Academy of Sciences of the United States of America·M SchenaR W Davis
Sep 8, 2000·Science·T A TatonR L Letsinger
Aug 31, 2002·Science·YunWei Charles CaoChad A Mirkin
Jan 6, 2004·Nature Biotechnology·Paul Alivisatos
Sep 17, 2004·Nature·Victor Ambros
Apr 24, 2008·Cancer Science·Shu TakakuraShunichi Yamashita
Aug 13, 2008·Nanomedicine·Y Charles Cao
Apr 16, 2009·Analytical and Bioanalytical Chemistry·Kyle A Cissell, Sapna K Deo
Feb 12, 2010·Methods : a Companion to Methods in Enzymology·Evan M KrohMuneesh Tewari
Mar 25, 2010·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Jill KoshiolMaria Teresa Landi
Apr 20, 2010·Angewandte Chemie·David A GiljohannChad A Mirkin
Mar 23, 2011·Biosensors & Bioelectronics·Zhiqiang Gao, Yanfen Peng

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Citations

Dec 2, 2016·Non-coding RNA·Joseph N Mwangi, Norman H L Chiu
Dec 4, 2020·Emerging Topics in Life Sciences·Ainoa GuinartTeresa D Tetley

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