Chiral HPLC analysis of venlafaxine metabolites in rat liver microsomal preparations after LPME extraction and application to an in vitro biotransformation study

Analytical and Bioanalytical Chemistry
Patrícia da Fonseca, P S Bonato

Abstract

A three-phase LPME (liquid-phase microextraction) method for the enantioselective analysis of venlafaxine (VF) metabolites (O-desmethylvenlafaxine (ODV) and N-desmethylvenlafaxine (NDV) in microsomal preparations is described for the first time. The assay involves the chiral HPLC separation of drug and metabolites using a Chiralpak AD column under normal-phase mode of elution and detection at 230 nm. The LPME procedure was optimized using multifactorial experiments and the following optimal condition was established: sample agitation at 1,750 rpm, 20 min of extraction, acetic acid 0.1 mol/L as acceptor phase, 1-octanol as organic phase and donor phase pH adjustment to 10.0. Under these conditions, the mean recoveries were 41% and 42% for (-)-(R)-ODV and (+)-(S)-ODV, respectively, and 47% and 48% for (-)-(R)-NDV and (+)-(S)-NDV, respectively. The method presented quantification limits of 200 ng/mL and it was linear over the concentration range of 200-5,000 ng/mL for all analytes. The validated method was employed to study the in vitro biotransformation of VF using rat liver microsomal fraction. The results demonstrated the enantioselective biotransformation of VF.

References

Aug 20, 2002·European Journal of Clinical Pharmacology·Marianne Gex-FabryGilles Bertschy
Jan 25, 2003·Pharmacogenetics·Chin B EapJacques Turgeon
Jun 7, 2003·Toxicology and Applied Pharmacology·Esther F A BrandonJan H M Schellens
Feb 1, 2005·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Stig Pedersen-Bjergaard, Knut Einar Rasmussen
Aug 4, 2006·Journal of Chromatographic Science·Tung Si HoKnut Einar Rasmussen
Dec 5, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Wen LiuHuan-de Li
Jun 3, 2008·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Patrícia da FonsecaPierina Sueli Bonato
Dec 18, 2008·Expert Review of Neurotherapeutics·Jayesh Kamath, Venkatesh Handratta
Feb 1, 2006·Environmental Toxicology and Pharmacology·M D Tingle, N A Helsby
Dec 1, 2004·Drug Discovery Today. Technologies·Robert J Riley, Ken Grime

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Citations

Jul 7, 2012·Biomedical Chromatography : BMC·Khalil FarhadiAmir Abbas Matin
Oct 31, 2014·Biomedical Chromatography : BMC·R Nageswara Rao, K Guru Prasad
May 6, 2020·Biomedical Chromatography : BMC·Gabriel HancuEnikő Barabás-Hajdu
Mar 16, 2013·Journal of Separation Science·Ewelina Dziurkowska, Marek Wesolowski
Jun 24, 2021·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·Yilin LiJiang Zheng

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