Chiral liquid chromatography tandem mass spectrometry in the determination of the configuration of glyceric acid in urine of patients with D-glyceric and L-glyceric acidurias

Biomedical Chromatography : BMC
M S RashedEmtethal Al-Jishi

Abstract

Glyceric acid is a highly polar chiral carboxylic acid that is usually not detected during routine organic acid analysis. Increased excretion is observed in two phenotypically distinct and rare inherited metabolic diseases, D-glyceric aciduria, and L-glyceric aciduria (also known as primary hyperoxaluria type 2). The determination of the exact configuration of the excreted glyceric acid is necessary for the accurate diagnosis of D-glyceric aciduria and for the differentiation between type 1 and type 2 primary hyperoxaluria. The separation of the two stereoisomers was achieved using a narrow-bore ristocetin A glycopeptide antibiotic silica gel bonded column. Triethylamine acetate at pH 4.1 with 10% methanol was used as mobile phase. The column was directly interfaced to a triple quadrupole tandem mass spectrometer and the electrospray ion source was operated in the negative ion mode. Three parent-to-daughter transitions were employed to specifically detect eluting glyceric enantiomers from essentially untreated urine samples. The two forms of glyceric acid were satisfactorily separated at 3.6 and 4.5 min. Application of the method led to the confirmation of three cases of D-glyceric aciduria from three different families. Two ot...Continue Reading

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Citations

Aug 8, 2015·Anais Da Academia Brasileira De Ciências·Nádia W DimerGustavo C Ferreira
Dec 6, 2017·Journal of Separation Science·Carlos CalderónMichael Lämmerhofer
Oct 16, 2010·Human Mutation·Jörn Oliver SassMelanie Walter
Sep 6, 2015·Annals of Clinical Biochemistry·Oliver Clifford-MobleyGill Rumsby
Jun 11, 2019·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Takahiro TakayamaKenichiro Todoroki

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