Chiral separation of gemifloxacin in sodium-containing media using chiral crown ether as a chiral selector by capillary and microchip electrophoresis

Electrophoresis
Seung Il ChoDoo Soo Chung

Abstract

Chiral crown ether, (+)-(18-crown-6)-tetracarboxylic acid (18C6H(4)), is an effective chiral selector for resolving enantiomeric primary amines owing to the difference in affinities between 18C6H(4) and each of the amine enantiomers. In addition to the destacking effect of sodium ion in the sample solution, the strong affinity of sodium ion to the polyether ring of crown ether is unfavorable to chiral capillary electrophoresis using 18C6H(4) as a chiral selector. In this report, the chiral separation of gemifloxacin dissolved in a saline sample matrix using 18C6H(4) was investigated. Adding a chelating agent, ethylenediaminetetraacetic acid (EDTA), to the run buffer greatly improved the separation efficiencies and peak shapes. The successful chiral separation of gemifloxacin in a urinary solution was demonstrated for both capillary and microchip electrophoresis.

References

Jan 1, 1996·Analytical Chemistry·L B KoutnyM Fuchs
Sep 1, 1997·Analytical Chemistry·A G HaddJ M Ramsey
Feb 12, 1998·Electrophoresis·C L ColyerD J Harrison
Feb 10, 1998·Electrophoresis·D Schmalzing, W Nashabeh
Aug 24, 1999·Analytical Biochemistry·C B CohenT T Nikiforov
Jan 14, 2000·Electrophoresis·J C McDonaldG M Whitesides
Jan 14, 2000·Electrophoresis·V DolníkS Jovanovich
Jan 14, 2000·Electrophoresis·I RodríguezS F Li
Feb 24, 2001·Electrophoresis·G Gübitz, M G Schmid
May 17, 2001·Antimicrobial Agents and Chemotherapy·G García-CalvoF Soriano
May 19, 2001·Electrophoresis·C J StewartD Perrett
Jun 14, 2001·Analytical Chemistry·N A Polson, M A Hayes
Dec 26, 2001·Chemical Reviews·R Vespalec, P Bocek
Feb 19, 2002·Chemical Reviews·József Szejtli
Mar 1, 1996·Analytical Chemistry·S C Jacobson, J M Ramsey

❮ Previous
Next ❯

Citations

Jul 16, 2005·The Analyst·Sarah A SheeleyJonathan V Sweedler
Jan 28, 2006·Angewandte Chemie·Detlev BelderManfred T Reetz
Jan 13, 2009·Journal of Separation Science·Guisen ZhangBi-Feng Liu
Jan 22, 2009·Electrophoresis·Raychelle M Burks, David S Hage
Feb 11, 2005·Journal of Chromatography. a·Seung Il ChoYong-Kweon Kim
May 17, 2017·Journal of Separation Science·Imran AliLeonid Asnin
Nov 10, 2005·Journal of Separation Science·Vladislav Dolnik, Shaorong Liu
Nov 27, 2004·Electrophoresis·Natalia PiehlDetlev Belder

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.