Chiral separation of rac-Ornidazole and detection of the impurity of (R)-Ornidazole in (S)-Ornidazole injection and raw material

Chirality
Jianquan HuangJunren Zhang

Abstract

(S)-Ornidazole is a subject of research as an antifertility agent in male animals at present. However, there seems to be no relative report on chiral separation for rac-Ornidazole, which has been used as an effective medicine for more than 30 years. In this article, the chiral separation of rac-Ornidazole on a Chiralcel OB-H column based on normal-phase high-performance liquid chromatography (NP-HPLC) is investigated and the methodology for detection of impurity of (R)-Ornidazole in (S)-Ornidazole injection and raw material is established. The novel mobile phase is utilized by mixing n-hexane, methanol and isopropyl alcohol (95:4:1, v/v/v) instead of the typical mobile phase of n-hexane and isopropyl alcohol, although the methanol, which offers a good resolution factor for the enantiomeric separation in this system, is not recommended on the Chiralcel OB-H column according to the instruction supplied by Daicel Chemical Ind., LTD (Japan).

References

Sep 1, 1979·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·D E SchwartzG Oesterhelt
Feb 1, 1984·International Journal of Andrology·D Stevenson, A R Jones
Jan 1, 1982·Australian Journal of Biological Sciences·D Stevenson, A R Jones
Jul 1, 1997·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·A R Jones, T G Cooper
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Feb 24, 2001·Journal of Chromatography. a·N M MaierW Lindner

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Citations

Mar 1, 2012·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Rui LiuLu-chuan Liu
Jun 24, 2014·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Yuran CaoYingyuan Zhang
May 22, 2007·Analytical Chemistry·R K Gilpin, C S Gilpin

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