Chiral separation of underivatized amino acids in supercritical fluid chromatography with chiral crown ether derived column

Chirality
Larry Miller, Lei Yue

Abstract

The supercritical fluid chromatographic separation of underivatized amino acids was explored using immobilized chiral crown ether column CROWNPAK CR-I (+) and mass spectrometric detection. The type of modifier, acidic additives, and the role of water were investigated. Enantioseparation was achieved for all 18 amino acids investigated with short retention times (less than 3 minutes) and average resolution of greater than 5.0. Analysis of enantiomerically pure standards demonstrated the D enantiomer eluted first for all amino acids using a CROWNPAK CR-I (+) column.

References

Apr 11, 2008·Journal of Separation Science·Debby Mangelings, Yvan Vander Heyden
Jul 25, 2015·Analytical Biochemistry·Hiroshi SugimotoFumihiro Jinno
Sep 1, 2015·Journal of Bioscience and Bioengineering·Yutaka KonyaEiichiro Fukusaki
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Mar 30, 2019·Analytical and Bioanalytical Chemistry·Adrien RaimbaultCaroline West
Oct 29, 2019·Analytical Chemistry·Daipayan RoyDaniel W Armstrong

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Citations

Oct 22, 2020·Journal of Chromatography. a·Mohammad Mahdi Moein, Thuy A Tran
Aug 8, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Róbert BerkeczIstván Ilisz

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