Chitin-like molecules associate with Cryptococcus neoformans glucuronoxylomannan to form a glycan complex with previously unknown properties.

Eukaryotic Cell
Caroline L RamosMarcio L Rodrigues

Abstract

In prior studies, we demonstrated that glucuronoxylomannan (GXM), the major capsular polysaccharide of the fungal pathogen Cryptococcus neoformans, interacts with chitin oligomers at the cell wall-capsule interface. The structural determinants regulating these carbohydrate-carbohydrate interactions, as well as the functions of these structures, have remained unknown. In this study, we demonstrate that glycan complexes composed of chitooligomers and GXM are formed during fungal growth and macrophage infection by C. neoformans. To investigate the required determinants for the assembly of chitin-GXM complexes, we developed a quantitative scanning electron microscopy-based method using different polysaccharide samples as inhibitors of the interaction of chitin with GXM. This assay revealed that chitin-GXM association involves noncovalent bonds and large GXM fibers and depends on the N-acetyl amino group of chitin. Carboxyl and O-acetyl groups of GXM are not required for polysaccharide-polysaccharide interactions. Glycan complex structures composed of cryptococcal GXM and chitin-derived oligomers were tested for their ability to induce pulmonary cytokines in mice. They were significantly more efficient than either GXM or chitin olig...Continue Reading

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Citations

Feb 28, 2014·Future Microbiology·Priscila C AlbuquerqueMarcio L Rodrigues
Sep 12, 2013·Future Microbiology·Anna VecchiarelliClaudia Monari
Jul 15, 2015·Scientific Reports·Jéssica RodriguesMarcio L Rodrigues
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Dec 18, 2013·Eukaryotic Cell·Juliana RizzoMarcio L Rodrigues
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Apr 13, 2018·Glycobiology·Zhuo A WangTamara L Doering
Feb 16, 2021·Frontiers in Cellular and Infection Microbiology·Diego de Souza GonçalvesAllan J Guimarães
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Mar 18, 2021·European Journal of Immunology·Keigo UenoYoshitsugu Miyazaki
Sep 28, 2021·Antimicrobial Agents and Chemotherapy·Alexandre Bezerra Conde FigueiredoMarcio L Rodrigues

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