Chitin purification from shrimp wastes by microbial deproteination and decalcification

Applied Microbiology and Biotechnology
Y XuJ Winter

Abstract

Chitin was purified from Penaeus monodon and Crangon crangon shells using a two-stage fermentation process with anaerobic deproteination followed by decalcification through homofermentative lactic acid fermentation. Deproteinating enrichment cultures from sewage sludge and ground meat (GM) were used with a proteolytic activity of 59 and 61 mg N l(-1) h(-1) with dried and 26 and 35 mg N l(-1) h(-1) with wet P. monodon shells. With 100 g wet cells of proteolytic bacteria per liter, protein removal was obtained in 42 h. An anaerobic spore-forming bacterium HP1 was isolated from enrichment GM. Its proteolytic activity was 76 U ml(-1) compared to 44 U ml(-1) of the consortium. Glucose was fermented with Lactobacillus casei MRS1 to lactic acid. At a pH of 3.6, calcium carbonate of the shells was solubilised. After deproteination and decalcification of P. monodon or C. crangon shells, the protein content was 5.8% or 6.7%, and the calcium content was 0.3% or 0.4%, respectively. The viscosity of the chitin from P. monodon and C. crangon was 45 and 135 mPa s, respectively, whereas purchased crab shell chitin (practical grade) had a viscosity of 21 mPa s, indicating a higher quality of biologically purified chitin.

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Citations

Apr 24, 2013·Applied Biochemistry and Biotechnology·Xiangzhao MaoHong Lin
Oct 30, 2012·Applied and Environmental Microbiology·Anna M KielakJan Dirk van Elsas
Oct 3, 2013·Critical Reviews in Biotechnology·Surinder Kaur, Gurpreet Singh Dhillon
Sep 28, 2018·Current Medicinal Chemistry·Md Iqbal Hassan KhanYonghao Ni
Sep 16, 2017·FEMS Microbiology Letters·Fernanda Kerche Paes da SilvaWolfram M Brück
Feb 6, 2020·Marine Drugs·Nathanael D ArnoldThomas B Brück
May 23, 2017·Frontiers in Microbiology·Gayathri IlangumaranBalakrishnan Prithiviraj
Dec 2, 2020·Environmental Pollution·Gincy Marina MathewAshok Pandey
Feb 7, 2021·Antioxidants·Manikandan MuthuIyyakkannu Sivanesan
Jul 23, 2021·3 Biotech·Gincy Marina MathewAshok Pandey

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