Chitin synthase B: a midgut-specific gene induced by insect hormones and involved in food intake in Bombyx mori larvae

Archives of Insect Biochemistry and Physiology
Weiwei ZhuoShiqing Xu

Abstract

Chitin synthase (CHS) is the key regulatory enzyme in chitin synthesis and excretion in insects, and a specific target of insecticides. We cloned a CHS B gene of Bombyx mori (BmChsB) and showed it to be midgut specific, highly expressed during the feeding process in the larva. Knockdown of BmChsB expression in the third-instar larvae increased the number of nonmolting and abnormally molting larvae. Exposure to nikkomycin Z, a CHS inhibitor, reduced the amount of chitin in the peritrophic membrane of molted larvae, whereas abnormally elevated BmChsB mRNA levels were readily detected from the end of molting and in the newly molted larvae. Exogenous 20-hydroxyecdysone (20E) and methoprene, a juvenile hormone analogue, significantly upregulated the expression of BmChsB when the levels of endogenous molting hormone (MH) were low and the levels of endogenous juvenile hormone (JH) were high immediately after molting. When levels of endogenous MH were high and those of endogenous JH were low during the molting stage, exogenous 20E did not upregulate BmChsB expression and exogenous methoprene upregulated it negligibly. When the endogenous hormone levels were low during the mulberry-leaf intake process, BmChsB expression was upregulated ...Continue Reading

Citations

Sep 1, 1993·Insect Biochemistry and Molecular Biology·K J KramerS Muthukrishnan
Jul 9, 1998·Insect Biochemistry and Molecular Biology·M G KimH Y Park
Sep 21, 2000·European Journal of Biochemistry·R L TellamR D Pearson
Nov 7, 2001·Pest Management Science·E Cohen
Jan 5, 2002·Insect Biochemistry and Molecular Biology·Mary Elizabeth GagouDimitris Kafetzopoulos
Jan 5, 2002·Insect Biochemistry and Molecular Biology·Michiyoshi TakahashiManabu Kamimura
May 31, 2002·Cell and Tissue Research·Lars Zimoch, Hans Merzendorfer
Jul 25, 2003·Insect Biochemistry and Molecular Biology·Takaaki DaimonToru Shimada
Nov 6, 2003·Insect Biochemistry and Molecular Biology·Keiko Takaki, S Sakurai
Aug 3, 2005·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Hans Merzendorfer
Oct 22, 2009·BMC Genomics·Michihiko ShimomuraKazuei Mita
Aug 5, 2011·Pest Management Science·Andrea J Bixby-Brosi, Daniel A Potter

Related Concepts

Serum Hormone Levels (Lab Test)
Molting
Chitin Synthetase 2
Chitin Biosynthetic Process
Bombyx
Nikkomycin
Analog
Citrullinemia
Larva
Gene Expression Regulation, Enzymologic

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