Chitin synthases are required for survival, fecundity and egg hatch in the red flour beetle, Tribolium castaneum

Insect Biochemistry and Molecular Biology
Yasuyuki ArakaneRichard W Beeman

Abstract

The synthesis of chitin, the beta-1,4-linked polymer of N-acetylglucosamine, is catalyzed by chitin synthase (CHS). Chitin is essential for the structural integrity of the exoskeletal cuticle and midgut peritrophic membrane (PM) of insects. To study the functions of the two chitin synthase genes, TcCHS-A and TcCHS-B, during embryonic and adult development in the red flour beetle, Tribolium castaneum, RNA interference (RNAi) experiments were carried out. When dsRNA for TcCHS-A was injected into male or female pharate adults, all insects died 5-7 d after the adult molt, and the females failed to oviposit prior to death. When dsTcCHS-A was injected into young adults 1-2 d post-eclosion, a similar lethal phenotype was obtained after 5 d and no oviposition occurred. When dsTcCHS-A injections were delayed until after adult maturation (7-10 d post-eclosion), the treated females did oviposit and the resulting embryos appeared to develop normally. However, the chitin content of the eggs was dramatically reduced, the embryos became twisted and enlarged, and the eggs did not hatch. Adults treated with dsRNA for TcCHS-B exhibited little or no chitin in their PM and died about 2 wk after injection. None of the TcCHS-B-treated females ovipos...Continue Reading

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Citations

Jun 21, 2013·Proceedings. Biological Sciences·Chris G C JacobsMaurijn van der Zee
Aug 13, 2013·PloS One·Jürgen DönitzGregor Bucher
Jan 5, 2014·PloS One·Alexandre Augusto Pereira FirminoMaria Fatima Grossi-de-Sa
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Jan 4, 2012·Insect Biochemistry and Molecular Biology·Hans MerzendorferSubbaratnam Muthukrishnan
Oct 6, 2010·Insect Biochemistry and Molecular Biology·Yasuyuki ArakaneRichard W Beeman
Feb 13, 2010·Insect Biochemistry and Molecular Biology·Yasuyuki ArakaneMichael R Kanost
Nov 10, 2009·Insect Biochemistry and Molecular Biology·Gunnar BroehanHans Merzendorfer
Mar 10, 2009·Insect Biochemistry and Molecular Biology·Yasuyuki ArakaneRichard W Beeman
Aug 21, 2013·Insect Science·Hans Merzendorfer
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Mar 10, 2017·Plant Biotechnology Journal·Caixiang LiuHuiru Tang

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